 Home > Biochemistry News > Plant Extracts News > American Chestnut [Castanea dentata (Marsh.) Borkh.]

American Chestnut [Castanea dentata (Marsh.) Borkh.]

 Last Update: 2021-01-13
 Source: Internet
 Author: User

Tags

saphris 10 mg

bisacodyl 10 mg

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

The key to successful transformation of American chestnut is having the correct combination of explant tissue, selectable and scorable markers, and a reliable regeneration system. Rapidly dividing somatic embryos, growing as proembryogenic masses, are a suitable tissue; the
bar
gene is a suitable selectable marker in conjunction with 1.0 to 10 mg/L phosphirothricin (PPT); and the
mgfp5-ER
gene is an effective nondestructive scorable marker. We have also found that the more gently the somatic embryos are treated during the inoculation and co-cultivation steps, the higher the transformation efficiency. The average transformation efficiency that can be expected using the described protocol is approx 20 stable and embryogenic transformation events/g of somatic embryo tissue. Cell line and batch-to-batch deviations both upward and downward should be expected. Finally, somatic embryos can be induced to form shoots, which can then be micropropagated and acclimatized.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Beta-Thujaplicin499-44-5

CAS No.: 499-44-5

API Grade

$100/KG EXW

Irinotecan hydrochloride CAS 100286-90-6

CAS No.: 100286-90-6

Pharmaceutical Grade

$100/KG EXW

Stachydrine hydrochloride CAS 4136-37-2

CAS No.: 4136-37-2

Pharmaceutical Grade

$100/KG EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.