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Many dicotyledonous and also several monocotyledonous plant species are susceptible to
Agrobacterium
-mediated transformation. This current and well-established method has been used successfully with a large number of plant species to mediate gene transfer. This chapter describes an
Agrobacterium
-mediated transformation method of
Petunia hybrida
leaf discs, one of the first species that was routinely transformed using this method.