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Advances in research on Arabidopsis genome assembly |
.
The researcher provided a schematic diagram of the Arabidopsis genome assembly process
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Researcher Professor Ye Kai and Associate Professor Yang Xiaofei of the School of Computer Science published research in the "Journal of Genome Proteomics and Bioinformatics" as co-corresponding authors.
Based on the experience accumulated in the assembly of opium poppy and human genome, they designed a comprehensive use of different sequencing technologies Advantageous hybrid sequencing strategy, proposed a new strategy for sequence replacement with bacterial artificial chromosomes as anchor points, and realized the high-quality Arabidopsis genome Col-XJTU with only two gaps .
Moreover, the genome has completed the gapless assembly of the centromeres of chromosomes 3, 4, and 5, and completed the assembly of most centromeres of chromosomes 1 and 2 .
In addition, the base accuracy and structural accuracy of this genome are both higher than the current reference genome TAIR10.
1.
The base accuracy is 99.
9999% .
https://doi.
org/10.
1016/j.
gpb.
2021.
08.
003