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Contamination of pathogenic bacteria in seawater threatens people's health and safety, and causes harm and losses to aquaculture.
the presence of a wide range of pathogenic bacteria in the marine environment may create synergies that highlight their potential threats, and some pathogenic bacteria are also a major repository of drug-resistant genes that play a role in the spread and evolution of drug-resistant genes.
, it is urgent to develop new methods for the rapid identification and detection of a variety of pathogenic bacteria in seawater.
Recently, Qin Wei, a researcher at Yantai Coastal Belt Research Institute of the Chinese Academy of Sciences, and his team proposed a new method for biometric sensing of polymer membrane electrodes based on the direct potential response of magnetically controlled biometric molecules, using the dual characteristics of highly selective identification and potential signal conduction of biometric molecules.
antimicrobial peptides as new identification molecules, not only can the identification of bacteria, but also the properties of their own ions can be used for the conduction of potential signals.
based on magnetically controlled polymer membrane potential sensing technology, the team identified molecules with antimicrobial peptides to achieve high sensitivity and high selectivity detection of Staphylococcus auspicillus, with a lower detection limit of 10 CFU mL-1.
researchers further selected four peptides as identification molecules, built a potential sensor array, through linear identification analysis, to achieve the classification of eight bacteria in the environmental sample (as shown).
The direct potential sensing technology of mark-free and indicator-free, which can be used for rapid electrochemical sensing analysis and identification of pollutants such as pathogenic bacteria in environmental water bodies, broadens the application range of polymer sensitive membrane potential sensors, and provides a new way of thinking for the direct potential analysis application of biometric molecules such as antibodies, peptides and nucleic acid fitters.
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