Absorption and metabolism of superoxide dismutase

2015-01-30 classification: efficacy 0 people commented that superoxide dismutase (SOD) is an active substance that can remove excessive free radicals in the body Cu Zn SOD was injected into human and animal blood by different ways, and the activity of exogenous SOD was determined by riboflavin staining Results the process of SOD in vivo was related to the way of injection If subcutaneous injection and intramuscular injection are used, the blood concentration can reach the peak within 2-3 hours After 6-7 hours, the blood concentration drops below 10% of the peak concentration If intravenous injection is used, the distribution half-life of SOD in the animal body is only 6-15 minutes, about 30 minutes in the human body, and 99% of SOD in th disappears from the body, indicating that the distribution and elimination of SOD in the body are rapid during intravenous injection 。 Radioimmunoassay and radioisotope labeling were used to study the metabolism of bovine blood Cu Zn SOD (35 μ g / g) The results showed that the concentration of SOD in kidney was the highest, followed by serum, lung and liver It was also found that exogenous SOD was mainly accumulated and inactivated in the renal cortex, i.e using a large dose (0.5mg / kg), the activity of SOD could not be detected in the urine of both human and animals, and there was no accumulation of SOD in vivo For patients with renal impairment or no kidney, the serum SOD level is very high, which may be due to the obstruction of SOD excretion, resulting in the accumulation of SOD in the body The results showed that SOD activity remained 81% when it was kept at 37 ℃ for 150 min in the simulated gastric acid of mice In the enzymolysis test of protease and pancreatin, SOD activity remained 82% and 8496% at 37 ℃ for 210 min after ligation of free small intestine, the permeability of SOD changed with time After 10 h, the total activity of SOD outside the small intestine was close to that in the small intestine when it was added into the small intestine Total activity The results showed that SOD could pass through intestinal mucosa Sod-pitg was obtained by labeling SOD with fluorescein isothiocyanate (pitg) After two hours of oral administration, the level of blood markers increased significantly, reaching the peak at four hours After four hours of oral administration of natural SOD, the level of blood SOD activity reached the peak Compared with the control group, the rate of activity increase was 30% - 40% The 1251 labeled SOD was given small The experiment of rat stomach perfusion also confirmed that the oral SOD can enter into human body At 3 hours after gavage, the peak of SOD was found in blood, most of SOD was distributed in plasma, and a small part of SOD was distributed in blood cells The distribution order of SOD in tissues was heart < spleen < liver < kidney.