A&R: Protein mannosylation as a diagnostic and prognostic biomarker for lupus nephritis

Purpose : Changes in protein glycosylation are a hallmark of immune- mediated diseases
.

Glycans major regulator of the inflammatory response, to distinguish between self - Important non-self molecules

ObjectiveObjective : protein glycosylation variations are immune sign mediated diseases

Methods : A comprehensive tissue glycomic characterization was performed in kidney specimens from patients with systemic lupus erythematosus and LN confirmed by biopsy .

Combined with advanced tissue mass spectrometry (matrix-assisted laser desorption ionization mass spectrometry imaging), in situ characterization of sugar (using PHA leukolectin (PHA-L) Evaluation β1,6 N - acetylglucosamine β1-6- branched N - For the expression of glycans, use snowdrop agglutinin (GNA) to evaluate the expression of mannosylated N - glycans) and phenotypic analysis of in vitro sugars, and perform structural map analysis on the N- glycan library in LN tissue samples .

MethodMethod : In from systemic lupus erythematosus and biopsy-proven LN conducted a comprehensive study to characterize tissue specimens sugar groups kidney patients

The resultsresult : LN exhibit unique glycan characteristics: high-mannose glycans and the abundance of space is usually only found in low microbe distribution increase

CONCLUSIONCONCLUSION : Cells mannose glycosylation LN marker, predictable CKD development, represents a potential biomarker sugar may be incorporated into LN diagnostic and prognostic algorithms

Alves, I.

, Santos-Pereira, B.
, Dalebout, H.

Alves, I.
, Santos-Pereira, B.
, Dalebout, H.
, et al.
(2021), Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.
Arthritis Rheumatol, 73: 2069-2077.
https://doi.
org/10.
1002/art.
41768 https://doi.
org/10.
1002/art.
41768 Leave a message here