-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
The nucleus is mainly composed of DNA, RNA and protein, and is the center of gene storage, replication and regulation
.
In the cell nucleus, biological macromolecules such as proteins form biomolecular aggregates through liquid-liquid phase separation (LLPS), which have been reported to be closely related to gene expression 1, chromatin higher structure 2, and so on
Recently, Ming Lei, Zhang Qiwei's research team at Tsinghua University School of Medicine/Beijing National Research Center for Information, collaborated with Li Tingting's research team at Peking University School of Basic Medicine and Chen Yang from the Institute of Basic Medicine, Chinese Academy of Medical Sciences to publish an online article "Quantifying" in Genome Biology.
The phase separation property of chromatin-associated proteins under physiological conditions using an anti-1,6-hexanediol index", developed a new method that can quantitatively describe the phase separation properties of chromatin-associated proteins under physiological conditions
.
In this work, the author developed a new method Hi-MS for enriching chromatin-related proteins based on the previously developed chromatin conformation capture technology BL-Hi-C 3
.
Preliminary experiments show that Hi-MS also has better sensitivity and specificity when using a lower cell mass (107)
1,6-Hexanediol (1,6-Hexanediol, 1,6-HD) has a moderate hydrophobic interaction interference ability, which can depolymerize the phase-separated aggregates without destroying the structure of the biofilm, and make Related proteins are separated from chromatin and are therefore widely used to identify the characteristics of protein phase separation
.
The author used the changes in the binding level of protein and chromatin before and after 1,6-HD treatment to define the AICAP (Anti-1,6-HD Index of Chromatin Associated Proteins) of chromatin-related proteins
AICAP represents the sensitivity of different proteins to 1,6-HD treatment.
For example, FUS, MED1 and other proteins have more detachment and have lower AICAP, while structural proteins such as histone and SMC-3 have less detachment and higher AICAP
.
In order to further explain the biological significance of AICAP, the author used bioinformatics analysis and found that AICAP is related to the phase separation characteristics of the protein (Figure 3)
.
Proteins with lower AICAP have a higher proportion of intrinsic disordered regions (IDR) and low complexity domains (LCD), and the disordered regions have more hydrophobic amino acids
The development of experimental methods for systematic identification and description of biological macromolecule aggregates and their components is very important for the development of the field of phase separation
.
In this study, the author newly developed Hi-MS technology, combined with Hi-C, described the changes in protein level and genome level after phase-separated aggregates were dissolved by 1,6-HD
Assistant researcher Shi Minglei, School of Medicine, Tsinghua University, is the co-first author and co-corresponding author of this paper.
You Kaiqiang, an eight-year doctoral student at Peking University School of Basic Medicine, is the co-first author of this paper.
Associate Professor Li Tingting, Professor Zhang Qiwei, Researcher Chen Yang is the co-corresponding author
.
Prof.
references:
1.
2.
3.
