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Recently, the Pearl River Fisheries Research Institute Song Hongmei "an amphilophus citrinellus black associate research fellow inventors of pigment separation and cell culture method of" national invention patents, patent number: ZL 2019 1 1342372.
1, 2021.
03.
23 Authorization
.
1, 2021.
03.
23 Authorization
.
The invention discloses a method for separating and culturing orange double-crowned cichlid melanocytes.
Trypsin and collagenase I are selected for co-processing, fish body surface tissues are predigested with trypsin, epidermal cells are removed, and collagenase I is used to prepare The collagenase solution is subjected to secondary treatment, combined with percoll of different titers and concentrations, to obtain pigment cells with higher viability
.
It has been successfully passaged for 10 generations, and the cells can maintain a good growth state, and can be frozen and recovered
.
Trypsin and collagenase I are selected for co-processing, fish body surface tissues are predigested with trypsin, epidermal cells are removed, and collagenase I is used to prepare The collagenase solution is subjected to secondary treatment, combined with percoll of different titers and concentrations, to obtain pigment cells with higher viability
.
It has been successfully passaged for 10 generations, and the cells can maintain a good growth state, and can be frozen and recovered
.
Animal pigment cells are important carriers for the study of animal body color formation
.
Therefore, the successful extraction, isolation and culture of pigment cells in vitro is the basis for exploring the biological functions of animal body color genes, as well as the prerequisite for understanding the molecular mechanism of fish body color formation and body color variation
.
In the prior art, researchers have successfully isolated and cultured melanocytes from the skins of mammals such as humans, mice, alpaca and foxes, but it is difficult to extract and separate pigment cells from the body surface of fish.
At present, no exact fish have been seen.
There are reports on the isolation and culture of certain pigment cells
.
In the present invention, after the melanocytes are separated, the melanocytes are observed through a transmission electron microscope.
The pterin and carotenoid vesicles inside the cells are complete, the internal structure of the cells is complete, and the pigment cell structure is complete, which must have good biological functions.
In order to use melanocytes cultured in vitro to study the molecular mechanism of fish body color formation, provide technical support and lay a theoretical foundation for fish body color molecular breeding
.
.
Therefore, the successful extraction, isolation and culture of pigment cells in vitro is the basis for exploring the biological functions of animal body color genes, as well as the prerequisite for understanding the molecular mechanism of fish body color formation and body color variation
.
In the prior art, researchers have successfully isolated and cultured melanocytes from the skins of mammals such as humans, mice, alpaca and foxes, but it is difficult to extract and separate pigment cells from the body surface of fish.
At present, no exact fish have been seen.
There are reports on the isolation and culture of certain pigment cells
.
In the present invention, after the melanocytes are separated, the melanocytes are observed through a transmission electron microscope.
The pterin and carotenoid vesicles inside the cells are complete, the internal structure of the cells is complete, and the pigment cell structure is complete, which must have good biological functions.
In order to use melanocytes cultured in vitro to study the molecular mechanism of fish body color formation, provide technical support and lay a theoretical foundation for fish body color molecular breeding
.
