A brief introduction to the vacuum centrifuge concentrator and its technical principles

Vacuum centrifugal concentrator has become one of the important instruments in food, biological, chemical laboratories, so how does the vacuum centrifugal concentrator work, what parts does it include, and how is it different from other concentration methods? In the later stage of the detection of biological samples, such as the use of electrophoresis, meteorological chromatography, high performance liquid chromatography, mass spectrometry and other methods, for experiments with high concentration requirements for sample concentration, you can use vacuum centrifugal concentration method to obtain a high concentration of samples for loading
.

Vacuum centrifugal concentration is particularly suitable for more sensitive biological samples, such as proteins, nucleic acids and other substances
.

Therefore, the main purpose of vacuum centrifugal concentration is to obtain a high concentration of sample without cross-contamination, sample loss, sample denaturation, reduced sample activity or oxidation
.

Compared with other concentration methods, the advantage of vacuum centrifugal concentration method is that the recovery rate of the sample is high, the harm to the environment is relatively small, and the operation of the general vacuum centrifugal concentrator is relatively simple, without complex experimental design.

The simple composition of the vacuum centrifuge concentrator generally includes three parts: centrifuge, connecting pipeline and vacuum pump, and the complete system includes: cold trap, flask, chemical trap and absorption column, vacuum control valve, vacuum manometer, trolley, etc
.
in addition to centrifuge, connecting pipeline and vacuum pump.

The principle of vacuum centrifugal concentration: the vacuum pump is used to form a vacuum in the concentrator, and Kia is reduced in the instrument, so that the boiling point of the solvent of the sample is reduced, and the solvent can boil and evaporate at low temperature; In addition, it can increase a certain amount of heat in the instrument cavity, thereby increasing the evaporation rate, but it will not affect the temperature of the sample, and the temperature of the sample itself will not increase; The sample will produce a small amount of centrifugal force at a suitable centrifugal speed, which prevents the loss caused by the sample bursting, and also avoids the phenomenon
of a large amount of foam in the centrifuge tube due to heating and centrifugation.