146 culture base formulations (bacterial and plant cultures)

culture
ingredients 1, Acetobacter Medium (Acetobacter Medium
Medium
)
Glucose (glucose) 100g Yeasst extract (
yeast
cream) 10g
CaCO3 20g Agar (
agar
) 15g
distilled water (steamed) Distilled water) 1000ml Adjust (tune) pH to 6.8
Scope of application: skunk Bacillus acetate turbid variant
2, Nutrient Agar (nutritional gravy agar)
Pepton (protein ) 5g Beef extract (beef paste) 30g
NaCl 5g Agar (agar) 15g
Distilled water (distilled water) Adjust (distilled water) Tune) pH to 7.0-7.2
When: When psinon of Bacillus, 5mg of to MnSO4.H2O may be added .
It is favorable to promote spore formation .
。 Scope of application: gas-producing Bacillus gas, fecal-producing Bacillus, waxy spores, waxy spores, herculin-shaped spores, giant spores, poly sticky spores, dust spores, sepsis, hot fat spores, dry Grass spores, dead grass spores deep black variants, Suyun golden spores, Suyun gold spores paraffin subseeds (Aocystil), Suyun gold spores Gorsd variants, Suyun gold spores inverted subsetopic species, the production of staphylococcus aureus, yellow staphylococcus aureus Bacteria, Glutamate Rod Bacillus, Beijing Bacillus, E. coli,E. coli, Copper Green Prosthetic Monocytobacteria (Green Pussy), Convex Prosthetic Monocytobacteria, Fluorescent Protons, Bending Protons, Skunk Monocytobacteria, Fake Monocytobacteria, Vine Yellow Eight Bacillus aureus, jaundia octaococcus, urea octaococcus, Staphylococcus aureus, sports fermentation monosporine
3, Azotobacter Medium (nitrogen fixation medium)
KH2PO4 0.2g K2HPO4 0.8g
MgSO4.7H2O 0.2g CaSO4.2H2O0.1g
。 Na2MoO4.2H2O Trace (micro) Yeast axtract (yeast paste) 0.5g
Mannitol (glycol) 20g FeCl3 Tract (Tract) Micro)
Distilled water (distilled water) 1000ml Agar (agar) 15g
Adjust (tune) pH to 7.2
Range: Nitrogen fixation bacteria, glial spores
4, Corn Meal Medium (cornmeal medium)
Maize flour (cornmeal) 5g Peptone (protein ) 0.1g
Glass (glucose) 1g Tap water (tap water) 1000ml
note: Boil the gas in autoclave at 121C for 1 hr. distribute the medium into 18x.18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121°C for 1 hr . again (15 pounds cooked for 1 hour, divided into 18x.18 mm
test tube
, each tube depth of up to 6 cm. 15 pounds sterilized again for 15 hours. )
5, Lactic-bacteria Medium I (Lactobacillus Media I)
Yeast extract (Yeast Cream) 7.5g Peptone (Protein ) 7.5g
Glucose (glucose) 10g KH2PO4 2g
Tomato juice (tomato juice) 100ml Tween (Twain) 80 0.5ml
distilled water (distilled water
Distilled water) 900ml pH 7.0
Application: Plant Lactobacillus (Lactobacillus Germ), Streptococcus hot lactic acid
6, Lactic-bacteria Midium II. (Lactobacillus lactobacillus culture) Base II.)
Lacto-casein peptone (cheese casein ) 10g Beef extract (protein ) 10g
Yeast extract (yeast cream) 5g Glucose (glucose) 5g
Tween (Twain) 80 1g K2HPO4 2g
Na-acetate (sodium acetate) 5g Diamine citrate (citric acid diamine) 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water (distilled water) 1000m pH 6.5 -6.8
For application: Plant Lactobacillus
7, Peotone Glucose Yeast Extract Medium PGY (protein, yeast paste, glucose medium
Peptone (protein ) 10g Yeast extract (yeast paste) 5g
Glucose (glucose) 1g Distilled water (distilled water) 1L
8, Glycerol Agar (glycerin agar)
Peptone (protein ) 5g Beef extract (yeast paste) 3g
Glycerol (glycerin) 20g Top water (tap water) 1 000ml
Agar (agar) 15g pH 7.0-7.2
9, Rhizobium medium (root tumor medium) AS 9
east eztract (yeast paste) 1g Soil eztract (soil leachate) 200ml
Mannitol (glycol) 10g Agar (agar) 15g
Distilled water (distilled water) 800ml pH 7.2
note: Soil extract: Suspend 50g finely and dried gardon soil in 200ml of tap water . Autoclave at 121°C for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121°C , then mixed with othringredients and distributed. (Soil leaching method: take 50 grams of soil, add water 200 ml, 15 pounds of cooking for 1 hour, after
filtration paper
filtration
after filling up to 200 ml of water.)
The scope of application: soybean root tumor bacteria (slow-life type), soybean slow root tumor bacteria, peanut root tumor bacteria, purple cloud English root tumor bacteria,
soybean root tumor bacteria (fast-forming type), soybean root tumor bacteria, pea root tumor bacteria, root tumor Bacillus, Tisseptin
10, Mannitol Agar (glycol agar)
Yeast extract (yeast paste) 5g Peptone (protein ) 3g
Mannitol (gan) Dew alcohol) 25g Agar (agar) 15g
Distilled water (distilled water) 1000ml
11, Glucose Asparagine (glucose, tianmen dongsin agar)
Glass (glucose) 10g Asparagine (Tianmen dong) 0.5g
K2HPO4 0.5g Water (water) 1000ml
pH 7.2-7.4
Scope of application: spore small monosporidium red variant, purple small monosporidium (red monosporidium)
12, Gause's Synthetic Agar (Gaussy Synthetic No. 1 agar)
KNO3 1 g Soluble starch 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
<3> Agar (agar) 20g water (water) 1000ml
pH 7.2-7.4
Scope of application: spore small monosporidium red variant, purple small monosporidium bacteria (red small monosporidium), white jaundial streptomycin White streptomycin, anti-streptomycin, double-roth streptomycin, color-producing streptomycin, ash streptomycin, sky blue streptomycin, mosquito streptomycin, erythromycin streptomycin, cyan streptomycin, cyan streptomycin, light gray streptomyceptia, Absorbent chain mold, light purple gray chain mold, yellow long spore chain mold, vine yellow chain mold, fine yellow chain mold, blackened chain mold, rose-colored chain mold, Huamei chain mold, hot chain mold, Venezuelan chain Mold, purple straight silk chain mold, purple chain mold, green chain mold
13, Wort Agar (malt juice agar)
Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word.. Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (Dilute the raw material of fermented beer (without hops) to 12 Berlin, add agar 15 grams, melt and sub-pack.) 15 pounds sterilized for 30 minutes.
scope of application: Cruise false silk yeast, Langbico false silk yeast, degreasing false silk yeast, Macedonian false silk yeast, tropical false silk yeast, stout false silk yeast, wrinkled false silk yeast, tropical false silk yeast, birth sclerosis yeast, asshu false sac yeast White mold, fruity mold, mould genus, abnormal Hanson yeast, abnormal Hanson yeast variant, Arabic glycol Hanson yeast, Shi's Hanson yeast, Kan sac yeast, Burton Bich yeast, membrane birch yeast, sticky red yeast, small red yeast, Gel red yeast, crimpen yeast, Carlsberg yeast, brewing yeast, coconut wine yeast, fermented yeast, Logan yeast, Ross yeast, Russ yeast polyformed Ruth yeast, Will yeast, yeast, Lutheran yeast, chestnut cracking yeast, throwing yeast Beres spore yeast, cortical spore yeast, brown spores, shell-free asromycin, fresh orange ascerlomy, erythromycin, erythromycin, red erythromycin
14, Potato Dextrose Agar PDA (potato, glucose agar)
Potato extract (potato juice) 1000ml Dextrose (glucose) 20g
Agar (agar) 20g
.Note: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until soft. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115 degrees C for 20 minutes. (Note: Remove the peeled potatoes 200g, cut them into small pieces, bring water 1000ml to the boil for 30 minutes, filter out the potato cubes, fill the filter to 1000ml, add 20g glucose, agar 15g, dissolve and sterilise for 30 minutes.)
Scope of application: Yeast rice noodles fake monocytobacteria (Yeast rice noodles aflatobacteria), white streptomycin, ash chain mold, cyan streptomyces, spore streptococcus, gray streptomyces, cracked chain mold, umbrella pear head mold, elegant radioactive hair Mold, rod-curd, mic, sprouted short-terrier mold, white stiff mold, grey grape spores, top spore mold, Brazilian wool shell, long prickly shell, olive-wrapped fur shell, anti-curved shell, amber shell, round enzyme hair shell, beam-like prickly spores, new moon bending spore mold, singular wing spore shell, ground wing spore shell, string bead spores, spores, plate spores (Lubao I), silver poplar plate spores, jade long creep, deep yellow spore mold, small spore mold, polycephaly spores, Raman Spore mold, grape-colored spore mold, raw fragrant mold, courgette mold, two types of spore mold, erect mold, spore mold, silk ball mold, large mold, polysporid mold, total hair mold, Lu's mold, five-way bridge