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Nature: Reveal the mechanism of action of glutamate transporters in cell membranes
Time of Update: 2021-03-24
Professor Renae Ryan, the co-corresponding author of the paper and the University of Sydney School of Medicine and Health, said, “Using cryo-EM, we have uncovered for the first time how glutamate transporters perform multi-tasking---performing dual functions, that is, in the cell membrane.
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Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
Time of Update: 2021-03-05
Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture.
In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
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Radioligand Binding Methods for Membrane Preparations and Intact Cells
Time of Update: 2021-03-04
The radioligand binding assay is a relatively simple but powerful tool for studying G protein-coupled receptors.
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Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells
Time of Update: 2021-03-03
Among the techniques available to assess Δ ψ , methods that rely on the spectrophotofluorometric responses of dyes are widely employed for whole suspensions of isolated mitochondria or permeabilized cells.
Here, we describe the use of safranine as a fluorescent indicator of Δ ψ in isolated mitochondria and digitonin-permeabilized cells.
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PNAS: Studies reveal the effects of cholesterol on cell membranes
Time of Update: 2021-02-17
These findings affect our understanding of the biological function of cholesterol and its role in health and disease.
"This goes against our understanding of the role of cholesterol in cell membranes," Ashkar said.
Misconceptions about how cholesterol enhances cell membranes affect our understanding of membrane function.
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Professors Deng Zeyuan and Li Hongyan of Nanchang University: Cell membrane ligands work together
Time of Update: 2021-02-10
Citation : , Deng Zeyuan, Li Hongyan, etc., of the National Key Laboratory of Food Science and Technology, Nanchang University School of Food, published the article Synergistic in the journal TOP Food
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Cell Death Differ: Single-cell RNA sequencing reveals heterogeneity in tumors of primary grape membrane melanoma
Time of Update: 2021-01-31
Single-celled RNA-seq revealed a poor prognosis of cell subgroups In this study used multiple-scale analysis using single-cell RNA sequencing of six different primary grape membrane melanomas, the researchers found heterogeneity in tumors at the genomic and transcriptional group levels.
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The permeability of the cell membrane
Time of Update: 2021-01-21
Understanding the entry of various substances into red blood cells Speed II, Small Molecule Cross-Membrane Transport Overview Biofilm transmetric penetration of small molecules includes water, electrolytes and non-electrolyte solutes.
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Membrane and Protein Dynamics in Virus-Infected Plant Cells
Time of Update: 2021-01-16
Therefore, to meet the challenges of diverse cell biology studies that will be derived from ongoing functional genomics projects, a series of methods relevant to the characterization of membrane and protein dynamics in virus-infected cells are provided here.
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Cell membrane camouflage helps the drug-carrying system evade the immune system
Time of Update: 2020-12-26
By the nano-carrier camouflaged by red blood cell membrane, using the complex biological composition and function of cell membrane, the successful "spoofing" of the body's immune system makes it a non-invasive source, smoothly through immune identification, extend the circulatory time in the blood, improve the effectiveness of drug supply in the lesions.
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Stroke: The lyic acid state of the cell membrane was negatively related to the prevalence of symptomatic cervical artery disease
Time of Update: 2020-12-17
, cell membrane-rich LA reflects its intake and is negatively associated with symptomatic cervical artery disease.
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Human amniotic membrane cells may be used for stroke treatment
Time of Update: 2020-12-16
study by researchers in Australia and other countries has found that injecting human amniotic cells into stroke patients can effectively reduce brain damage and help patients recover.
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Flow Cytometric Analysis of Cell Membrane Microparticles
Time of Update: 2020-12-16
Cell membrane microparticles (MPs) are phospholipid microvesicles shed from the plasma membrane of most eukaryotic cells undergoing activation or apoptosis.
MPs have been analyzed in plasma and cell cultures by means of flow cytometry or solid phase assays.
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Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes
Time of Update: 2020-12-10
Artificial lipid bilayers can be used to mimic cell-to-cell interactions, but a major challenge remains how to tether molecules to these membranes.
This allows us to study the interaction of HEL-specific B cells with the tethered antigen by a variety of microscopy techniques.
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A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular
Time of Update: 2020-12-06
Therefore, we examined the effects of cholesterol removal by utilizing our current methods on the hydrogen peroxide (H 2 O 2 )-activated PLD in BPAECs. Differences in the loss of cholesterol and the resulting effects on the cell membrane, cell viability, morphology, and the extent of oxidant-induced PLD activation were determined.