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G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings
Time of Update: 2020-11-16
In order for us to understand the initial and persisting effects of UV in seedlings, we “delay” light-induced development by carrying out all experiments in complete darkness between days 0 (seed) and day 7 (Arabidopsis).
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Champs-Elyses
Time of Update: 2020-11-16
Form: , 20m high, chest diameter 2m; The scaly leaves are aromatic, the leaves on the main branch have glands, and those on the side twigs have no glands or are very small.
Columna: Canopy column, 4 to 5m high; 'egg circle' champs cv.
Hoveyi: Egg canopy, 1.5m high.
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Gramene Database: A Hub for Comparative Plant Genomics
Time of Update: 2020-11-16
The rich collection of known genetic information and the recent completion of rice genome sequencing project provided the cereal plant researchers a useful tool to investigate the roles of genes and genomic organization that contribute to numerous agronomic traits.
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Dahurian Angelica
Time of Update: 2020-11-16
The seeding period is from late August to early September, when the hole is planted at a distance of 35×15-20cm, and the seed is spread evenly by the line distance of 35cm, and then thin soil is covered.
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Protein Blotting Protocol for Beginners
Time of Update: 2020-11-16
The transfer and immobilization of biological macromolecules onto solid nitrocellulose or nylon (polyvinylidene difluoride (PVDF)) membranes subsequently followed by specific detection is referred to as blotting.
With few exceptions, western blotting involves five steps, namely, sample collection, preparation, separation, immobilization, and detection.
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Nitrate and Nitrite
Time of Update: 2020-11-16
Young animals and babies are particularly at risk because the small volume of blood that they contain requires only a small amount of nitrite to convert all the hemoglobin to methemoglobin.
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Determining Abundance of MicroRNAs and Other Small RNAs in Legumes
Time of Update: 2020-11-16
We highlight the advantages and shortcomings of each method and offer a detailed description of those techniques that best work in our hands, in particular having in mind their use for the study of several small RNAs in a given sample.
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Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay
Time of Update: 2020-11-16
Regulatory DNA sequences harbor the essential information to control specific gene expression changes and integrate information derived from upstream signaling cascades.
The analysis of these DNA regions, at which several signaling pathways could merge to orchestrate gene expression, is still a challenging task.
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Assay of Photoinhibition of Photosystem II and Protease Activity
Time of Update: 2020-11-16
Under light-stress conditions, the photosystem (PS)II reaction center D1 protein is photodamaged.
The method to analyze the protease activity in the stroma that recognizes and digests the crosslinked products of the D1 protein generated by the light stress is also described.
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Elan
Time of Update: 2020-11-16
The fruit is purple and black berries, round or long eggs, containing 3 to 12 brown, flat round seeds, ripe from December to March of each year.
Annual flower production varies with tree age, climate and cultivation management, with a typical single plant producing 5 to 10 kg.
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Spruce.
Time of Update: 2020-11-16
Sichuan spruce trees per hectare average yield of about 40 kg, generally 9 to 10 moon fruit ripening, seeds spread by wind distance is about 2 to 3 times the height of the mother tree.
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Pollen Cultivation and Preparation for Proteomic Studies
Time of Update: 2020-11-16
Optimized protocols for pollen collection, storage, and in vitro culture as well as pollen organelle separations are described which help to obtain well-defined and reproducible experimental conditions for the subsequent proteomic analysis.
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A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination
Time of Update: 2020-11-16
Chemical genetics approach, in which bioactive small molecules are used to perturb the function of gene products encoded by functionally redundant genes, provides an excellent alternative strategy to investigate ABA signaling.
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Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling
Time of Update: 2020-11-16
LM has become an established strategy in many areas of biology and it has recently been adapted for use with many types of plant tissue.
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An Efficient Method for the Micropropagation of Agave Species
Time of Update: 2020-11-16
Micropropagation offers an alternative to this problem through the efficient cloning of selected high-yielding “elite” plants.
We report here efficient method to micropropagate agaves and a strategy for the management of large scale production that has been successfully applied to severalAgave spp.
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Beer and Wort Proteomics
Time of Update: 2020-11-16
Proteome analysis provides a way to identify proteins related to the quality traits of beer.
In addition, low molecular weight polypeptides in beer have been identified by the combination of non-enzyme digestion and mass analyses.
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A Non-targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding
Time of Update: 2020-11-16
Upon wounding or herbivory, plants quickly react by activating various defense mechanisms.
Here, we describe a method based on ultrahigh-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry that can potentially profile the full range of known free and esterified jasmonates in a non-targeted manner.
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Immunomodulation of Plant Function by In Vitro Selected Single-Chain Fv Intrabodies
Time of Update: 2020-11-16
This needs basic prerequisites to be successfully applied as resources and techniques to isolate specific recombinant antibodies with sufficient binding parameters to bind and to block even low-concentrated targets or to compete successfully with substrates and ligands.
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Micropropagation and Cryopreservation of Garlic (Allium sativum L.)
Time of Update: 2020-11-16
Micropropagation is used for speeding up the vegetative propagation mainly using the advantage to produce higher numbers of healthy plants free of viruses, which have higher yield than infected material.
The main micropropagation methods rely on growth of already formed meristems.
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Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays
Time of Update: 2020-11-16
A prerequisite for this change was an efficient and reliable protocol for the isolation of chloroplasts for use in protein import assays, enabling biochemical approaches to be combined with the genetic potential of the plant.
