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TagFinder: Preprocessing Software for the Fingerprinting and the Profiling of Gas ChromatographyMass Spectrometry Based Metabolome Analyses
Time of Update: 2020-11-17
As GC-MS technology represents one essential element which contributes to investigations of substantial equivalence, we have developed a dedicated software tool, the TagFinder chromatography data preprocessing suite, which has all essential functions to support both fundamental workflows of modern metabolomic studies.
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Chemical Genetic Analysis of Protein Kinase Function in Plants
Time of Update: 2020-11-17
Identification of protein kinase targets and specific inhibition of individual kinase isoforms on the protein levelin planta are important techniques to elucidate signal transduction pathways.
The use of ATP-binding pocket mutants, the so-called gatekeeper mutants, that accommodate N 6 -enlarged nucleotides and kinase inhibitors has allowed a dramatic increase in kinase isoform selectivity.
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Pinus densiflora
Time of Update: 2020-11-16
Leaf needle shape, 2 a bunch, length 8 to 12 cm, softer, diameter about 1 mm (oil pine needle) Leaf diameter 1.5 to 2 mm), resin tube 6 to 7 , side-born, with cesamic leaves.
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T-DNA Transfer to Maize Plants
Time of Update: 2020-11-16
Agrobacterium tumefaciens is a soil bacterium that induces, in most dicotyledonous plants, the neoplastic disease called crown gall.
Additional T-DNA genes encode enzymes that produce, in the gall, novel amino acid and sugar derivatives, called opines, which are specific growth substrates for the bacterium.
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Applications of Ultra-high-Throughput Sequencing
Time of Update: 2020-11-16
Applications of ultra-high-throughput sequencing (UHTS) are essentially limited only by the imaginations of researchers, and include genome sequencing/resequencing, small RNA discovery, deep SNP discovery, chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) coupled with sequence identification, transcriptome analysis including empirical annotation, discovery and characterization of alternative splicing, and gene expression profiling.
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The Age of Protein Kinases
Time of Update: 2020-11-16
In this introductory chapter, we briefly review general definitions, methodology, and current concepts of the molecular mechanisms of protein kinase function as a foundation for this methods book.
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make a gentleman
Time of Update: 2020-11-16
plant is 2 to 8 meters high.
Umbrella room-shaped spiked flower sequence set of raw branches, May to September flowering, corolla white at first, then turn pale red, beautiful and aromatic.
Tropical areas are often used as ornamental plants or hedges.
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Transcriptome Profiling Analysis Using Rice Oligonucleotide Microarrays
Time of Update: 2020-11-16
Transcriptome analysis using oligonucleotide microarrays is a powerful tool for detecting changes in genome-wide transcripts under a given biological condition.
Genome-wide transcriptome analysis is a useful tool for elucidating the functions of rice genes that have not yet been determined.
Here, we introduce methods for genome-wide transcriptome analysis in rice.
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Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis
Time of Update: 2020-11-16
Time-resolved hypocotyl length measurements in seedlings have the potential to greatly aid genetic studies looking at light, hormone, and circadian regulation of cell expansion.
Recently, several computer-based tools have been developed to quantify hypocotyl length during photomorphogenesis and early seedling development.
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New Technologies for Ultra-High Throughput Genotyping in Plants
Time of Update: 2020-11-16
Two forms of sequence-based marker, simple sequence repeats (SSRs), also known as microsatellites and single nucleotide polymorphisms (SNPs) now predominate applications in modern plant genetic analysis, along the anonymous marker systems such as amplified fragment length polymorphisms (AFLPs) and diversity array technology (DArT).
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Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus
Time of Update: 2020-11-16
Phytoplasma collections are a vital resource for researchers and diagnosticians studying phytoplasma diseases.
They provide material as a point of reference and a research tool to increase our understanding of phytoplasmas and the diseases they cause.
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Metabolomics Protocols for Filamentous Fungi
Time of Update: 2020-11-16
Proteomics and transcriptomics are established functional genomics tools commonly used to study filamentous fungi.
Here, we describe broad generic protocols that can be used to undertake metabolomics studies in filamentous fungi.
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Medicago truncatula Transformation Using Root Explants
Time of Update: 2020-11-16
Medicago truncatula is a forage crop that has been developed into a model legume.
The development of different transformation methods is an important aspect for functional genomic studies in the species.
Furthermore, the protocol can be easily adapted for recovering fertile transgenic plants from transformed hairy roots.
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Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS
Time of Update: 2020-11-16
Plant metabolic pathways and the molecular and atomic fluxes through them can be deduced using stable isotopically labeled substrates.
Here, we summarize the application of gas chromatography-time of flight mass spectrometry (GC-TOF-MS) for metabolic studies using differently labeled elemental isotopes applied to both intact organelles and whole plant tissue.
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Meristem-Tip Culture
Time of Update: 2020-11-16
Typically, the explant is between 3-4 mm and 2 cm in length, and development in vitro is still regulated so as to be confined to outgrowth of an organized shoot, without adventitious propagation.
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Mineral Composition Analysis: Measuring Anion Uptake and Anion Concentrations in Plant Tissues
Time of Update: 2020-11-16
This chapter describes two basic complementary methods relevant to at least three major macronutrients in plants: NO3 − , SO4 2− , and phosphate.
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Agrobacterium-Mediated Transformation to Create an Insertion Library in Magnaporthe grisea
Time of Update: 2020-11-16
Polyethylene glycol/CaCl 2 -mediated protoplast transformation was the initial method we used and resulted in the generation of just more than 17,000 insertion strain lines.
LaterAgrobacterium tumefaciens -mediated transformation was adopted and the final number of insertional mutant strains ofM.
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Elan
Time of Update: 2020-11-16
The fruit is purple and black berries, round or long eggs, containing 3 to 12 brown, flat round seeds, ripe from December to March of each year.
Annual flower production varies with tree age, climate and cultivation management, with a typical single plant producing 5 to 10 kg.
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Reconstructing Plant Cells in 3D by Serial Section Electron Tomography
Time of Update: 2020-11-16
Tomography has been used to visualize cells in 3D, and single particle analysis has been used to investigate macromolecules and viral particles.
In this chapter we will describe how we have collected tilting series micrographs from plant cells and how we have reconstructed the cellular volumes using dual axis electron tomography.
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Automated DNA Extraction for Large Numbers of Plant Samples
Time of Update: 2020-11-16
The method described here is a rapid, totalDNA extraction procedure applicable to a large number of plant samples requiring pathogen detection.
The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads.
