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Muskmelon Embryo Rescue Techniques Using In Vitro Embryo Culture
Time of Update: 2020-11-07
Unfortunately, the commercial melon varieties are not able to produce viable hybrids when crossed with their wild melon counterparts.
In vitro melon embryo rescue has played a main role to obtain viable hybrids originated from commercial versus wild melon crosses.
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Metasequoia.
Time of Update: 2020-11-07
It was not until 1946 that it was confirmed that the trees were water fir, a plant that had lived on Earth hundreds of millions of years earlier.
well-known living fossil plants in China are vultures, silver fir, water pine, Taiwan fir, money pine and so on.
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The eucalyptus tree
Time of Update: 2020-11-07
Chinese ancient books have long been cultivated eucalyptus trees recorded, many places today still keep a small number of large trees.
, the eucalyptus tree, which belongs to the same genus, has a long history of cultivation in China.
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Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II
Time of Update: 2020-11-07
The protocols regarding separation and purification of LHCII subcomplexes by non-denaturing isoelectric focusing (ndIEF), confirmation of trimeric nature of the subcomplexes, and establishing their p
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ZhouPi
Time of Update: 2020-11-07
When the roots and stems grow coarsely, the cells of the secondary tissue wood hydrant formation layer divide vertically, forming radially arranged cell columns, which are differentiated outward into the wood hydrant layer and inward into the inner layer of the hydrant.
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Challenges and Approaches to Statistical Design and Inference in High-Dimensional Investigations
Time of Update: 2020-11-07
The novel and challenging biological questions asked from HDE data have resulted in many specialized analytic techniques being developed.
This chapter discusses some of the unique statistical challenges facing investigators studying high-dimensional biology and describes some approaches being developed by statistical scientists.
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Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells
Time of Update: 2020-11-07
In particular, changes in free polyamine levels and in the activities of related enzymes seem to be linked to different growth kinetics (cell division and/or root elongation) depending on the different degree of expression of T- DNA genes.
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Sugar cane (Saccharum officinarum)
Time of Update: 2020-11-07
From the temperature limit of the sugar cane area, the average annual temperature of 17 degrees C or more, the most suitable temperature is 30 to 35 degrees C, soil moisture needs 70 to 80%, high fertilizer consumption, is a high-light four carbon plants, high productivity of dry matter, there is "energy crops."
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Purification of EffectorTarget Protein Complexes via Transient Expression in Nicotiana benthamiana
Time of Update: 2020-11-07
Analyses of genome sequences revealed that oomycete pathogens, such asPhytophthora spp., possess hundreds of RXLR effectors that are thought to be delivered into the host cells and hence function inside the cells by interacting with the host protein complexes.
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Mapping In Vivo ProteinDNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method
Time of Update: 2020-11-07
DamID, an alternative method to chromatin immunoprecipitation (ChIP), is based on the covalent linking of a “fingerprint” in the vicinity of the DNA-binding sites of the protein of interest.
thaliana (3).
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Sesame.
Time of Update: 2020-11-07
the fat content of sesame seeds in about 55%, protein content of about 25%, containing amino acids up to 17 kinds, the human body essential methionine, glutamate and other 7 amino acids content is relatively rich.
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Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins
Time of Update: 2020-11-07
This technique requires the labelling of proteins by fluorochromes before their separation on 2DE gels.
This technique may be applied to a wide array of plant stress studies.
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Amplified Fragment Length Polymorphism: An Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic Studies
Time of Update: 2020-11-07
Amplified fragment length polymorphism (AFLP) is aPCR -based technique that uses selective amplification of a subset of digestedDNA fragments to generate and compare unique fingerprints for genomes of interest.
Variations of standard AFLP methodology have been also developed for targeting additional levels of diversity, such as transcriptomic variation and DNA methylation polymorphism.
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Liu.
Time of Update: 2020-11-07
Common willows, small branches slender sagging.
Dry willow twigs are not sagging, yellow or green, smooth; the leaves are sharp at the beginning, the base wedges are or nearly round, the edges are noticeably finely jagged, green on top, gray-white below.
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Mesh grass class
Time of Update: 2020-11-07
Leaf cross to life, egg-shaped or oval shape, stems, leaf handles, flower stalks are dense hair, its characteristics are leaf-faced dense red or white mesh, strong resistance to negative, in addition to winter to early spring easy to encounter cold rotting leaves, other seasons of leaves do not wither, quite fresh and elegant.
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From Proteomics to Systems Biology: MAPA, MASS WESTERN, PROMEX, and COVAIN as a User-Oriented Platform
Time of Update: 2020-11-07
Examples for the integration of MAPA and MASS WESTERN data, proteogenomic and metabolic modeling approaches for functional genomics, phosphoproteomics by integration of MOAC (metal-oxide affinity chromatography) with MAPA, and the integration of metabolomics, transcriptomics, proteomics, and physiological data using this platform are presented.
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Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts
Time of Update: 2020-11-07
It details preparation of protoplasts fromArabidopsis suspension cultured cells or leaves of soil-grownArabidopsis plants and subsequent PEG-mediated transformation with reporter and effector plasmids.
This protocol can be completed within 24 h from protoplast preparation to reporter assay.
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Analysis of miRNA Modifications
Time of Update: 2020-11-07
One function of this modification is to protect miRNAs from 3′ terminal uridylation by an unknown enzymatic activity.
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Maize (Zea mays L.)
Time of Update: 2020-11-07
Agrobacterium tumefaciens -mediated transformation is an effective method for introducing genes into maize.
In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II.
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millet
Time of Update: 2020-11-07
is commonly known as millet in northern China, while rice is called millet in the south.
There is also an African-born valley, also known as Pearl Millet, which is often confused with millet in English.
