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Soilless cultivation of commonly used substititures - rice shells
Time of Update: 2020-11-09
Carbonized rice shell weight 0.15g/cm 3 , total porosity 82.5%, large pore 57.5%, small pore 25.0%, water-to-air ratio of 1:0.43, pH 6.5.
After the carbonization of rice shell, pH is often more than 9.0, must be washed or adjusted with acid after use, in order to ensure the normal growth and development of crops.
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Fungicide Resistance Assays for Fungal Plant Pathogens
Time of Update: 2020-11-09
Laboratory assays are used to determine loss of sensitivity, or resistance, to a fungicide and can explain fungicide failures and for developing successful fungicide recommendations in the field.
Laboratory assays for fungicide resistance are conducted by measuring reductions in growth or spore germination of fungi in the presence of fungicide, or by molecular procedures.
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Calla.
Time of Update: 2020-11-08
(1) White terrier horseshoe lotus tubers are smaller and grow more slowly.
(1) Green terrier horseshoe lotus tubers are large, strong growth, late flowering.
(2) Cultivated horseshoe lotus usually plants balls after autumn.
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Anchovies
Time of Update: 2020-11-08
Sub-plants, in February-March between the selection of one or two years of birth mother bamboo 3-5 plants for a clump of soil planting.
Long-term maintenance of pot soil moist, half-yin conservation, diligent spray water to the leaf surface, fertilization once a month.
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MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary Metabolism
Time of Update: 2020-11-08
MeRy-B contains lists of plant metabolites, mostly primary metabolites and unknown compounds, with information about experimental conditions, the factors studied, and metabolite concentrations for 19 different plant species (Arabidopsis, broccoli, daphne, grape, maize, barrel clover, melon,Ostreococcus tauri , palm date, palm tree, peach, pine tree, eucalyptus, plantain rice, strawberry, sugar beet, tomato, vanilla), compiled from more than 2,300 annotated NMR profiles for various organs or tissues deposited by 30 different private or public contributors in September 2013.
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Cucumber (Cucumis sativus L.)
Time of Update: 2020-11-08
The second involves regeneration from a long-term established embryogenic suspension culture emitting green autofluorescence (GAF) and selection on medium containing hygromycin.
(No false positives were observed.) The transformation efficiency in the leaf microexplants fluctuated from 0.8 to 6.5% of the primary explants, whereas in the embryogenic suspension-cultured cells it varied from 6.4 to 17.9% of the aggregates.
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Inter stock
Time of Update: 2020-11-08
The conventional cultivation process of seedlings is to choose the local suitable apple real birch as the base, graft the dwarfed birch near the ground, and then graft the apple variety at 25-35cm in the dwarfed middle branch the following year, ceding to produce the seedlings.
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Optical Trapping in Plant Cells
Time of Update: 2020-11-08
Optical tweezers allow noninvasive manipulation of subcellular compartments to study their physical interactions and attachments.
In this chapter, we provide practical tips for setting up such experiments paying special attention to the technical considerations for integrating optical tweezers into a confocal microscope.
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Use of Transcriptomics to Analyze Chloroplast Processes in Arabidopsis
Time of Update: 2020-11-08
The most powerful approach to the analysis of the transcriptional regulation of chloroplast functions involves RNA hybridization to microarrays representing almost all nuclear genes ofArabidopsis thaliana , followed by statistical data analysis.
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Production of Plantibodies in Nicotiana Plants
Time of Update: 2020-11-08
In this chapter, the procedures from the isolation of the monoclonal antibody genes to the biochemical and biological characterization of the plant-expressed monoclonal antibody are described.
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Detection of Programmed Cell Death in Plant Embryos
Time of Update: 2020-11-08
As in most if not all the developmental cell deaths in plants, embryonic PCD is hallmarked by autophagic degradation of the cytoplasm and nuclear disassembly that includes breakdown of the nuclear envelope andDNA fragmentation.
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Micropropagation of Pear (Pyrus sp.)
Time of Update: 2020-11-08
Elements of micropropagation include establishment of shoot tip cultures, proliferation, rooting, and acclimatization of the resulting plantlets.
Pear shoots are often recalcitrant to rooting; however, a 5 s dip in 10 mM indole-3-butyric acid or naphthalene acetic acid before planting on basal medium without plant growth regulators is effective for many genotypes.
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Two-Dimensional Nanoflow Liquid Chromatography-Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots
Time of Update: 2020-11-08
The protocol involves harvesting of leaves and roots from rice plants, preparing protein extracts from the harvested tissues, preparing proteolytic digests of the extracted proteins, making a biphasic capillary column with an integral electrospray emitter, performing two-dimensional chromatographic separation of peptides with data-dependent tandem mass spectrometry, and the use of database searching of the acquired tandem mass spectra to identify peptides and proteins.
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A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii
Time of Update: 2020-11-08
Chlamydomonas reinhardtii is also a favorable organism for chloroplast transformation because it contains a single chloroplast and grows heterotrophically when supplemented with acetate.
Chlamydomonas has served as a model organism for the development of chloroplast transformation procedures and the study of photosynthetic mutants generated using this method.
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Eight fairy flowers
Time of Update: 2020-11-08
Sub-plants are carried out before early spring germination, cutting the top branches and taking root 2 weeks after inserting them.
Potted plants can properly shade the sun and extend the flowering period.
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Crops are prepared for sexual interbreeding
Time of Update: 2020-11-08
Generally for short-term sun crops such as late rice and soybeans, from the seedling period to the spike before flowering, shorten the daily lighting time, can promote flowering, extend the daily lighting time, can delay flowering.
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Spindle tree
Time of Update: 2020-11-08
After the rainy season, the arrival of the dry season, green leaves have withered, red flowers have opened, at this time, a spindle tree has become a large vase with red flowers, so people call it bottle tree.
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A leafy orchid
Time of Update: 2020-11-08
In the long-term domestic demand for sufficient moisture, cultivation environment needs to be wet, summer needs to be maintained under the shade, winter at more than 0 degrees C can be safe winter.
A leafy orchid is more resistant, and stained water is susceptible to pests and diseases.
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A High-Throughput Biological Conversion Assay for Determining Lignocellulosic Quality
Time of Update: 2020-11-08
Lignocellulosic biomass is a source of low cost polysaccharides that some microbes can deconstruct and convert into liquid transportation fuel.
Here we describe a small-scale high-throughput assay that measures ethanol production from a culture of plant biomass and the ethanologenClostridium phytofermentans .
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Computational Identification of Candidate Nucleotide Cyclases in Higher Plants
Time of Update: 2020-11-08
In higher plants guanylyl cyclases (GCs) and adenylyl cyclases (ACs) cannot be identified using BLAST homology searches based on annotated cyclic nucleotide cyclases (CNCs) of prokaryotes, lower eukaryotes, or animals.
We foresee that the application of this method can be used to identify many more members of the growing family of CNCs in higher plants.
