"Zebrafish embryo test acute toxicity of natural plant extract" obtained invention patent

On October 14, it was learned that Chen Feng, a researcher of South China Botanical Garden, Chinese Academy of Sciences, had obtained the national patent for invention (Patent No.: zl201410687308.8) for the "method for testing the acute toxicity of natural plant extracts by using zebrafish embryos" The invention relates to a method for testing the acute toxicity of natural plant extract by using zebrafish embryo, which comprises the following steps: preparing embryo culture medium, dissolving the plant extract powder with dimethyl sulfoxide to clarification, filtering twice The final concentration of DMSO in the diluent with different concentrations of plant extracts was the same and less than or equal to 1.2% (V / V) Zebrafish embryos were cultured with different concentrations of diluent, and then the effects of different concentrations of diluent on zebrafish embryo development were observed and recorded at 7-72 HPF to determine the toxicity of plant extracts on zebrafish embryo development The method has the advantages of simple operation, strong practicability, preliminary judgment of acute toxicity of plant extract, fast speed and high accuracy Editor: ye Ruiyou news source: www.cas.cn/syky/201610/t20161014_.shtml Special statement: this reprint is only for the needs of science popularization and information dissemination, which does not mean that it represents the opinions of this website or confirms the authenticity of its content; if other media, websites or individuals reprint from this website, they must keep the "source" indicated on this website, and bear the legal responsibilities such as edition rights; if the author does not want to be reprinted or other related matters, please contact us 。