Yiqiao Shenzhou quickly listed Lambda and Delta plus recombinant proteins

In the current COVID-19 pandemic, the phrase "Delta is not flat, Lambda is up again"

From reference 1

01 Lambda (C.

On June 14, 2021, the WHO announced that this new crown mutant strain Lambda was listed as a "Variants of Interest, VOI" (Variants of Interest, VOI).

According to the information provided by the WHO, the Spike protein of the Lambda mutant strain has mutation sites such as G75V, T76I, del247/253, L452Q, F490S, D614G, and T859N.

Lambda and Delta coincidentally both produced mutations at position L452, which may indicate that mutations at this position may have caused the rapid spread of these two viruses

According to the results published in the preprint bioRxiv by researchers from the University of Tokyo on July 28, two key viral characteristics of the Lambda mutant strain were found:

1.

2.

From reference 2

In addition, under the background that the vaccine has been widely vaccinated, people are very concerned about whether the virus can escape the protective effect produced by the vaccine.

However, the Lambda mutant strain has just emerged, and there are fewer relevant studies and reports, and more experiments are needed by scientific researchers in order to have a more in-depth understanding of this potentially high-risk mutant strain

Although the lack of a large segment of S-NTD has not yet become a mainstream change

Study on important mutation sites of Lambda

Molecule label

Mutation site

N, His

P13L, R203K, G204R, G214C

RBD, His

L452Q, F490S

S1+S2 ECD, His

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G, T859N

40589-V08B24

(In development)

S1+S2 ECD, His

G75V, T76I, R246del, S247del, Y248del, L249del, T250del, P251del, G252del, D253N, L452Q, F490S, D614G, T859N

40591-V08H31

(In development)

S1, His

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G

40592-V49H6-B

(In development)

RBD, His & AVI

L452Q, F490S

Study on important mutation sites of Lambda

Study on important mutation sites of Lambda

Study on important mutation sites of Lambda

Item No.

Molecule label

Mutation site

Item No.

Item No.

Molecule label

Molecule label

Mutation site

Mutation site

40588-V07E31

N, His

P13L, R203K, G204R, G214C

40588-V07E31

40588-V07E31

N, His

N, His

P13L, R203K, G204R, G214C

P13L, R203K, G204R, G214C

40592-V08H113

RBD, His

L452Q, F490S

40592-V08H113

40592-V08H113

RBD, His

RBD, His

L452Q, F490S

L452Q, F490S

40589-V08B23 (under development)

S1+S2 ECD, His

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G, T859N

40589-V08B23 (under development)

40589-V08B23 (under development)

S1+S2 ECD, His

S1+S2 ECD, His

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G, T859N

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G, T859N

40589-V08B24

(In development)

S1+S2 ECD, His

G75V, T76I, R246del, S247del, Y248del, L249del, T250del, P251del, G252del, D253N, L452Q, F490S, D614G, T859N

40589-V08B24

(In development)

40589-V08B24

(In development)

S1+S2 ECD, His

S1+S2 ECD, His

G75V, T76I, R246del, S247del, Y248del, L249del, T250del, P251del, G252del, D253N, L452Q, F490S, D614G, T859N

G75V, T76I, R246del, S247del, Y248del, L249del, T250del, P251del, G252del, D253N, L452Q, F490S, D614G, T859N

40591-V08H31

(In development)

S1, His

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G

40591-V08H31

(In development)

40591-V08H31

(In development)

S1, His

S1, His

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G

G75V, T76I, R246N, Δ247, Δ253, L452Q, F490S, D614G

40592-V49H6-B

(In development)

RBD, His & AVI

L452Q, F490S

40592-V49H6-B

(In development)

40592-V49H6-B

(In development)

RBD, His & AVI

RBD, His & AVI

L452Q, F490S

L452Q, F490S

02 Delta and Delta plus

At present, the most concerned about the world is undoubtedly the Delta mutant strain.
Since the Delta mutant strain was discovered in India at the end of last year, it has rapidly swept the world and became the main epidemic strain in most parts of the world
.
And many studies have shown that its infectious ability has greatly increased, and the protective effect of vaccines has also been significantly reduced
.

The WHO has redefined the Delta mutation, and four lineages including B.
1.
617.
2, AY.
1, AY.
2, and AY.
3 are considered to be strains of concern
.
The two lineages AY.
1 and AY.
2, which introduced the K417N mutation in Delta, are named Delta plus
.
And K417N already exists in the previous Beta mutant strain
.

Study on important mutation sites of Delta and Delta plus

Item No.

Molecule label

Mutation site

40588-V07E32

N, His

D63G, R203M, G215C, D377Y

40592-V08H115

RBD, His

K417N, L452R, T478K

40592-V49H5-B

RBD, His & AVI

K417N, L452R, T478K

40589-V08B25

(In development)

S1+S2 ECD, His

T19R, G142D, W258L, K417N, L452R, T478KD614G, D950N, P681R

40589-V08B26

(In development)

S1+S2 ECD, His

T19R, V70F, E156G, del157/158, A222V, K417N, L452R, T478K, D614G, P681R, D950N

40589-V08B27

(In development)

S1+S2 ECD, His

T19R, E156G, del157/158, L452R, T478K, D614G, P681R, D950N

Study on important mutation sites of Delta and Delta plus

Study on important mutation sites of Delta and Delta plus

Study on important mutation sites of Delta and Delta plus

Item No.

Molecule label

Mutation site

Item No.

Item No.

Molecule label

Molecule label

Mutation site

Mutation site

40588-V07E32

N, His

D63G, R203M, G215C, D377Y

40588-V07E32

40588-V07E32

N, His

N, His

D63G, R203M, G215C, D377Y

D63G, R203M, G215C, D377Y

40592-V08H115

RBD, His

K417N, L452R, T478K

40592-V08H115

40592-V08H115

RBD, His

RBD, His

K417N, L452R, T478K

K417N, L452R, T478K

40592-V49H5-B

RBD, His & AVI

K417N, L452R, T478K

40592-V49H5-B

40592-V49H5-B

RBD, His & AVI

RBD, His & AVI

K417N, L452R, T478K

K417N, L452R, T478K

40589-V08B25

(In development)

S1+S2 ECD, His

T19R, G142D, W258L, K417N, L452R, T478KD614G, D950N, P681R

40589-V08B25

(In development)

40589-V08B25

(In development)

S1+S2 ECD, His

S1+S2 ECD, His

T19R, G142D, W258L, K417N, L452R, T478KD614G, D950N, P681R

T19R, G142D, W258L, K417N, L452R, T478KD614G, D950N, P681R

40589-V08B26

(In development)

S1+S2 ECD, His

T19R, V70F, E156G, del157/158, A222V, K417N, L452R, T478K, D614G, P681R, D950N

40589-V08B26

(In development)

40589-V08B26

(In development)

S1+S2 ECD, His

S1+S2 ECD, His

T19R, V70F, E156G, del157/158, A222V, K417N, L452R, T478K, D614G, P681R, D950N

T19R, V70F, E156G, del157/158, A222V, K417N, L452R, T478K, D614G, P681R, D950N

40589-V08B27

(In development)

S1+S2 ECD, His

T19R, E156G, del157/158, L452R, T478K, D614G, P681R, D950N

40589-V08B27

(In development)

40589-V08B27

(In development)

S1+S2 ECD, His

S1+S2 ECD, His

T19R, E156G, del157/158, L452R, T478K, D614G, P681R, D950N

T19R, E156G, del157/158, L452R, T478K, D614G, P681R, D950N

Quality verification

Lambda and Delta plus recombinant protein products are available from stock, and have been verified by SDS-PAGE and ELISA activity
.

Lambda-RBD-His (L452Q, F490S):

Cat: 40592-V08H113

Purity:> 95% as determined by SDS-PAGE

Activity: binding affinity EC50 is 17.
7ng/mL

Delta plus-RBD-His (K417N, L452R, T478K):

Cat: 40592-V08H115

Purity:> 95% as determined by SDS-PAGE

Activity: binding affinity EC50 is 32ng/mL

Reference materials:

[1]Comparison of Neutralizing Antibody Titers Elicited by mRNA and Adenoviral Vector Vaccine against SARS-CoV-2 Variants

[2]SARS-CoV-2 Lambda variant exhibits higher infectivity and immune resistance.