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Wuhan Institute of Virology/Biosafety Science Center has made progress in the research of multi-site editing of large DNA virus genomes

 Last Update: 2022-01-08
 Source: Internet
 Author: User

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Recently, the international academic journal ACS Synthetic Biology ("ACS Synthetic Biology") published online the latest research results of the team of Hu Zhihong and Wang Manli of Wuhan Institute of Virology, Chinese Academy of Sciences/Biosafety Science Research Center
.
The paper is entitled "Multiloci manipulation of baculovirus genome reveals the pivotal role of homologous regions in viral DNA replication, progeny production, and enhancing transcription"
.
The study used synthetic biology technology to edit the 8 homologous repeat regions (hrs) of insect baculoviruses at multiple sites, revealing the function of hrs
.

Viral genome editing is a key technology for basic and applied research in virology
.
Multi-target manipulation of the genomes of large DNA viruses such as baculovirus, herpesvirus, and poxvirus is technically challenging, and the editing of repetitive sequences in the viral genome is even more difficult
.
Many large DNA viruses have multiple copies of homologous repeat regions (hrs), which play a key role in the life cycle of the virus
.
In this study, the baculovirus artificial synthesis platform constructed in the early stage was used to delete the genome of Autographa californica nuclear polyhedrosis virus (AcMNPV) at multiple sites at the same time, and artificially synthesized the AcMNPV genome Syn-Δhrs with all hrs removed, and A single hr1, hr2, or hr3 Syn-hr1, Syn-hr2, Syn-hr3 were retained, and the virus rescue was successfully carried out
.
Research results show that hrs is not necessary for virus replication, but it can enhance gene transcription in adjacent regions, and the transcription enhancement effect is related to the specific sequence of hrs
.
The above research proves the feasibility and superiority of synthetic biology technology in multi-site editing and functional research of large DNA viruses, and also provides new ideas for the optimal design of baculovirus expression vectors
.

Figure: Synthesis, rescue and transcriptome analysis of hr deletion baculovirus

This research was funded by the National Natural Science Foundation of China (31872640) and the Frontier Science Key Research Project of the Chinese Academy of Sciences (QYZDJ-SSW-SMC021)
.
Hu Hengrui, a doctoral student at Wuhan Institute of Virology, is the first author of the paper, and researchers Hu Zhihong and Wang Manli are the co-corresponding authors of the paper
.

Paper link: https://pubs.
acs.
org/doi/full/10.
1021/acssynbio.
1c00303

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