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Fruit sugar accumulation is an important agronomic trait
of horticultural crops, especially fruit trees.
Sugar determines the flavor, taste and nutritional quality of the fruit; In addition, it can also be used as a regulatory signal to promote the synthesis
of secondary metabolites such as anthocyanins.
As an important economic fruit tree in China, most of the cultivars of peach are mainly accumulated sucrose (up to 80% of the total soluble sugar).
The insufficient separation of fruit sugar phenotypes in cultivars (soluble solids between 11%~14%), which limited the number of
sugar candidate genes isolated by forward genetics methods.
In order to reveal the mechanism of this important agronomic trait, the scientific research team of molecular breeding of fruit trees in Wuhan Botanical Garden of the Chinese Academy of Sciences selected wild relatives with large phenotypic differences from cultivated varieties as controls, and used transcriptome and sugar component content coupling analysis methods to explore key candidate genes
for sugar accumulation.
of horticultural crops, especially fruit trees.
Sugar determines the flavor, taste and nutritional quality of the fruit; In addition, it can also be used as a regulatory signal to promote the synthesis
of secondary metabolites such as anthocyanins.
As an important economic fruit tree in China, most of the cultivars of peach are mainly accumulated sucrose (up to 80% of the total soluble sugar).
The insufficient separation of fruit sugar phenotypes in cultivars (soluble solids between 11%~14%), which limited the number of
sugar candidate genes isolated by forward genetics methods.
In order to reveal the mechanism of this important agronomic trait, the scientific research team of molecular breeding of fruit trees in Wuhan Botanical Garden of the Chinese Academy of Sciences selected wild relatives with large phenotypic differences from cultivated varieties as controls, and used transcriptome and sugar component content coupling analysis methods to explore key candidate genes
for sugar accumulation.
The content of fruit sugar components in different developmental stages of peach varieties ('American Late Oil', 'Ligrant', 'Xiahui No.
6', 'Xia Crisp' and Mountain Peach, etc.
) was determined by high performance liquid chromatography, and transcriptome sequencing was performed in the three fruit development stages of these varieties, and the differences in gene expression were compared with the differences in sugar components to obtain differential gene sets
positively and negatively correlated with total sugars.
6', 'Xia Crisp' and Mountain Peach, etc.
) was determined by high performance liquid chromatography, and transcriptome sequencing was performed in the three fruit development stages of these varieties, and the differences in gene expression were compared with the differences in sugar components to obtain differential gene sets
positively and negatively correlated with total sugars.
Among the 424 differential genes positively associated with total sugar, the PpTST1 vacuole membrane sugar transporter gene at the top of chromosome 5 was previously reported, and two SPS (sucrose-phosphate synthase) genes and SuSy1 (sucrose synthetase) genes were found in this study, and the function of related genes was verified by the transient transformation method of
peach fruit.
Among the genes positively and negatively correlated with the total sugar content of peach fruit, one INH glycosic acid invertase inhibitory peptide gene was found, namely PpINH3 (total sugar positive correlation) and PpINHa (total sugar negative correlation) genes
.
The instantaneous conversion proves that these two INHs have the opposite effect on the accumulation of total sugars, and the transient conversion of the previously reported INH substrate acid invertase PpVIN2 has the same
effect as PpINHa.
However, in this study, the yeast biheterogeneous could not identify the molecular interaction between INH and PpVIN2
.
The mechanism of action of INH needs to be further studied
.
peach fruit.
Among the genes positively and negatively correlated with the total sugar content of peach fruit, one INH glycosic acid invertase inhibitory peptide gene was found, namely PpINH3 (total sugar positive correlation) and PpINHa (total sugar negative correlation) genes
.
The instantaneous conversion proves that these two INHs have the opposite effect on the accumulation of total sugars, and the transient conversion of the previously reported INH substrate acid invertase PpVIN2 has the same
effect as PpINHa.
However, in this study, the yeast biheterogeneous could not identify the molecular interaction between INH and PpVIN2
.
The mechanism of action of INH needs to be further studied
.
In addition, this study also found the presence of another sucrose synthetase SuSy2
in the candidate gene negatively associated with total sugar.
In addition, there are two monosaccharide transporters, PpERD6-Like1 and PpERD6-Like2, which are transient overexpression that may cause changes in the expression of PpTST1
.
Based on the above findings, the pattern of fruit sugar accumulation in peach (Figure 4) was obtained, and it was found that fruit sugar accumulation in peach originated both from metabolism and source-library transport in the body, and was also regulated
by various factors.
This study was published in the international research journal Frontiers in Plant Science, supported by the National Natural Science Foundation of China and other projects, and the first authors of the paper are Md Dulal Ali Mollah and Zhang Xian, Bangladeshi students, and Associate Professor Wang Lu and Professor Han Yuepeng are the corresponding authors
of the paper.
This study reveals the important genes and mechanisms in the accumulation of sugar in peach fruit, provides research examples and technical elements for the fine regulation of fruit sugar components, and opens up new ideas
in the development of functional healthy fruits.
in the candidate gene negatively associated with total sugar.
In addition, there are two monosaccharide transporters, PpERD6-Like1 and PpERD6-Like2, which are transient overexpression that may cause changes in the expression of PpTST1
.
Based on the above findings, the pattern of fruit sugar accumulation in peach (Figure 4) was obtained, and it was found that fruit sugar accumulation in peach originated both from metabolism and source-library transport in the body, and was also regulated
by various factors.
This study was published in the international research journal Frontiers in Plant Science, supported by the National Natural Science Foundation of China and other projects, and the first authors of the paper are Md Dulal Ali Mollah and Zhang Xian, Bangladeshi students, and Associate Professor Wang Lu and Professor Han Yuepeng are the corresponding authors
of the paper.
This study reveals the important genes and mechanisms in the accumulation of sugar in peach fruit, provides research examples and technical elements for the fine regulation of fruit sugar components, and opens up new ideas
in the development of functional healthy fruits.
