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Ion pair reagent is a special reagent for high performance liquid chromatography.
When using liquid chromatography to analyze samples with relatively strong ionization ability, the retention time of the sample on the reversed-phase chromatographic column is very short or not retained at all.
The type and concentration of ion pair reagents have a greater impact on the separation effect
Alkyl sulfonate and sulfonate have similar application objects, but the selectivity is different.
Commonly used quaternary ammonium ion pair reagents mainly refer to quaternary ammonium salts containing C4-C30, such as tetrabutylammonium hydroxide (10% aqueous solution), tetrabutylammonium bromide, dodecyltrimethylammonium chloride, etc.
In the selection of ion pair reagents, it should be noted that the type and concentration of ion pair reagents have a greater impact on the separation effect.
Related Links: Ion Pair Reagent