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The RNA-guided CRISPR/Cas9 system is the simplest and most efficient gene editing tool, but the site it can edit needs to contain a sequence called PAM, which limits its editing scope
Wang Yongming's research group is committed to developing a small Cas9 system, expanding the scope of editing, and filling the domestic gap
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| Figure 1. |
More than 300 Cas9s have been identified, and a series of tool enzymes with excellent performance have been developed
On August 12, 2022, the research group made another breakthrough and published a research paper entitled "Closely related type II-C Cas9 orthologs recognize diverse PAMs" online on eLife
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| Figure 2. |
Subsequently, the research team delved into Nsp2Cas9, which recognizes N 4 CC PAM and has editing activity comparable to that of Nme2Cas9
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| Figure 3. |
On this basis, the authors developed Nsp2-SmuCas9 chimera, which recognizes N 4 C PAM, the simplest Cas9 for PAM ( Figure 4 )
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| Figure 4. |
Doctoral student Wei Jingjing is the first author of the paper, and Professor Wang Yongming and Deputy Chief Physician Sun Shuna of the Pediatric Hospital of Fudan University are the co-corresponding authors of the paper
Release date: 2022/8/15
