 Home > Biochemistry News > Plant Extracts News > Visual Selection in Rice: A Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products

Visual Selection in Rice: A Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products

 Last Update: 2021-01-16
 Source: Internet
 Author: User

Tags

high pressure pneumatics

red fluorescent dye

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Fluorescent proteins such as green fluorescent protein (GFP) allow direct visualization of transformed cells without the need for exogenous substrates. Furthermore, visual selection using GFP is a powerful tool that can be used to isolate transformed cells without antibiotic or herbicide pressure and can be applied to transformation systems in plants hypersensitive to these agents. Moreover, we propose that visual selection enables isolation of calli in which the gene of interest is expressed to a high level, by selecting calli in which a strong GFP signal is observed. However, until now, the efficiency of clonal propagation using visual selection has been lower than that in antibiotic selection because of the technical difficulties involved in the isolation and clonal propagation of transformed calli with conventional transformation frequencies. We have succeeded in improving the efficiency of clonal propagation by the use of a rice cultivar that exhibits high competency for
Agrobacterium
-mediated transformation.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Premium ingredient High purity Resveratrol

CAS No.: 501-36-0

food grade

$10-12/KG FOB

Apigenin / 520-36-5

CAS No.: 520-36-5

pharmaceutical Grade

$10/KG FOB

Fisetin / 528-48-3

CAS No.: 528-48-3

pharmaceutical Grade

$10/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.