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Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules

 Last Update: 2020-11-17
 Source: Internet
 Author: User

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Virus-induced gene silencing (VIGS) has emerged as a powerful method for studying gene function. VIGS is induced by infecting a plant with a plant virus that has had its genome modified to include a sequence from the host gene to be silenced.
DNA
β and DNA1 are satellite and single-stranded DNA molecules associated with begomoviruses (family
Geminiviridae
). We converted DNAβ and DNA1 into gene-silencing vectors. The VIGS vectors can induce silencing efficiently in many solanaceous plants. Here, we describe procedures for the use of these two gene-silencing vectors for VIGS in different hosts.

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