Vaccines can be vaccinated by cupping. Scientists use ancient methods and modern methods to increase delivery efficiency by nearly 100 times

Cupping is a traditional Chinese medicine treatment method.

Recently, a researcher has developed a new vaccination method, which uses a method similar to cupping to deliver DNA directly through the skin and increases the delivery efficiency by nearly 100 times.

DOI: 10.

In the past 20 years, nucleic acid drugs have been extensively developed and applied.

The mechanism of nucleic acid drugs is to allow synthetic or engineered nucleic acids to enter host cells, and then express and export the encoded protein, for example, waiting for processing by antigen-presenting cells after vaccine injection, thereby inducing an immune response

In vivo nucleic acid transfection is divided into two ways: viral and non-viral

Nonetheless, these devices are expensive and require specialized training, which limits large-scale applications

In this study, a research team from Rutgers University in the United States and GeneOne Life Science, a South Korean biopharmaceutical company, first injected the new crown vaccine into mice intracutaneously.

GFP expression under dermal aspiration

Then the researchers used a single variable method to study the dependence of pressure, treatment time, DNA amount and device type on GFP expression.

The dependence of GFP expression on pressure, treatment time, DNA amount and device type

In addition, the researchers also stated that “negative pressure not only enables the non-invasive distribution of DNA into tissues, but also produces a powerful transfection effect, allowing molecules to cross the cell membrane barrier into the cell.

All in all, this study shows a safe and effective skin transfection method, which can not only carry out high-level transgene expression, but also has no tissue damage and obvious adverse reactions in animal immune models

Reference materials:

[1] DOI: 10.