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Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration

 Last Update: 2021-01-15
 Source: Internet
 Author: User

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gene expression pogil

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Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in
Medicago truncatula
leaves using
Agrobacterium tumefaciens
infiltration. Using quantitative real-time
PCR
we demonstrate that the infiltration of a construct containing the
LEGUME ANTHOCYANIN PRODUCTION 1
(
LAP1
) transcription factor results in the strong upregulation of key biosynthetic genes and the accumulation of anthocyanin pigment in the leaves after just 3 days. Thus, this method provides a rapid and powerful way to the discovery of downstream targets of
M. truncatula
transcription factors.

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