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Transcriptome analysis using oligonucleotide microarrays is a powerful tool for detecting changes in genome-wide transcripts under a given biological condition. Although the rice genome sequence is available, the number of functionally characterized genes in rice is still very limited. Genome-wide transcriptome analysis is a useful tool for elucidating the functions of rice genes that have not yet been determined. Currently, more than 3,000 arrays are publicly available. Here, we introduce methods for genome-wide transcriptome analysis in rice.