To study and analyze the new mechanism of double-folding of mouse monolithic stem cells

p53 knocked out of the monolithic stem cells in a variety of differentiation experiments outside the body, all showed a very strong single-fold maintenance ability, bioinfox analysis found that the stability of the monolithic maintenance and apoptosis gene inhibition.
On June 4th, Researcher Shuai Shuai of Nanca University's National Key Laboratory of Pharmaceutical Chemistry Biology, in a paper published online in Stem Cell Report by Yu Yang, a researcher at The Third Hospital of Peking University, and Fan Yong, a researcher at the Third Hospital of Guangzhou Medical University, found that inhibition of apoptosis through gene regulation can reduce the double-folding of mouse monomatopenic embryonic stem cells in the process of daily culture and differentiation. The findings provide a quick and effective strategy for obtaining various heterolyx differentiated cells, and promote genetic screening studies of thromosome cells in various genealogy.
the establishment of hemnogenic embryonic stem cells provides a new means for genetic screening research and preparation of animal models for gene editing, and is a valuable genetic research tool. However, in the process of daily cultivation and differentiation, it often doubles the phenomenon, which seriously affects its function of "gene screening device". How to inhibit double and obtain monolithic embryonic stem cells efficiently has become an important subject in stem cell research.
of the genetic information of nuclear life comes from the parent and half from the mother. Naturally, therefore, there are two sets of chromosomes in the cell, which is also the way mammals transmit genetic information, and by subtracting division to resist environmental changes and ensure species reproduction. However, for genetic research, especially the exploration of the function of recessive genetic genes, due to the existence of allogens, due to the existence of secondary cells are limited.
two sets of chromosomes are equivalent to 'double insurance', and if one of the genes is regulated, the altered gene cannot immediately be highlighted due to the presence of a corresponding allele 'backup'. However, there is only one set of chromosome monolithic cells, and without a gene 'back-up', our changes to any gene directly lead to esoteric changes. This is very beneficial for us to explore the phenomenon of life and crack the genetic code. As a result, heteosome cells are known as "gene screening devices."
the past decade, heliopolyte stem cells of several species, including mice, rats, monkeys and humans, have appeared one after another, which has attracted wide attention in the fields of genetic screening and drug target analysis.
because polypic stem cells have the characteristics of pure hydration esoteric and stem cell polydifferent potential, it is available to quickly apply polypic stem cells to explore unknown functions of novel genes. However, in the path of daily culture or differentiation, tool cells of this type of artificial pure thymogene tend to spontaneously double, thereby losing the advantage of a single-copy genome.
, the need to rely on time-intensive and complex selection methods for periodic collection of entogeneity cells, which has brought great limitations to the application of monolithic stem cells in many fields. It is of far-reaching significance to explore the mechanism of double-folding of monolithic stem cells, so as to maintain the single-factority of monolithic stem cells fundamentally and effectively, and to promote the tool cells of such genetic screening research, Shuai said.
researchers found that knocking out the p53 gene in the genome can stabilize the single-fold of mouse hematopoietic embryonic stem cells. Experiments show that p53 knocked out mouse heteropotential stem cells in the in-body differentiation, can obtain a monolithic high purity of the anthroposome, upper embryo stem cell-like cells and neural stem cell-like cells. The in vivo differentiation experiments showed that p53 knocked out mouse hemplate embryonic stem cells can contribute to embryonic development day 6.5, day 8.5 and day 10.5 through chiplate experiments, and maintain a high monoplate ratio. In addition, monolithic cells can also be detected in differentiation experiments in teratomas.
To explore the reasons why p53 knocked mouse heptosome embryonic stem cells remained stable and single-fold, the researchers found that p53 knocked out mouse monolithic embryonic stem cells, apoptosis gene expression decreased significantly, while p53-related other signaling path pathfound gene expression levels did not see significant differences. Subsequent results show that the absence of apoptosis gene p73 can also effectively maintain the single-fold of mouse hematopoietic embryonic stem cells.
" study proved that p53 knockout is mainly by inhibiting apoptosis, to maintain the single-fold of mouse hematopoietic embryonic stem cells in the process of daily transmission culture and internal and external differentiation. This provides a fast and effective strategy for obtaining various thromosome differentiated cells, and also popularizes the genetic screening of thromosome cells in various genealogy. The handsome leader said. (Source: Xinyu Wu Junhui, China Science Daily)
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