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Time-Profiling Fluorescent Reporters in the Arabidopsis Root

 Last Update: 2020-11-20
 Source: Internet
 Author: User

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Confocal laser scanning microscopy is a useful nondestructive approach for the visualization of fluorescent reporters
in planta
. Samples are usually placed between a slide and a cover slip which, although suited to single time-point imaging, does not allow long-term observation. Here, we describe a technique to monitor changes in fluorescence in the
Arabidopsis
root over a long period of time. Treatment can easily be performed, and this approach is suitable for use in low-throughput chemical screens. We also present a rapid method to analyze fluorescence intensity profiles generated using this protocol.

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