 Home > Biochemistry News > Biotechnology News > Three elements of Taq enzyme selection: amplification efficiency, enzyme motility, and sensitivity

Three elements of Taq enzyme selection: amplification efficiency, enzyme motility, and sensitivity

 Last Update: 2022-09-14
 Source: Internet
 Author: User

Tags

low temperature paint

multiplex pcr

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Guide

At present, there are many brands of hot-start Taq enzyme sold in the domestic market, but there are not many really high-quality hot-start Taq enzymes, in the face of so many hot-start Taq enzyme products, how should we choose?

First, warm-start Taq enzymes with high amplification efficiency are selected

After the well-tolerated Taq enzyme reaction system is optimized, the amplification efficiency is more than 95%, and the amplification can be obtained satisfactorily even when the content of the target gene is low, and it is not easy to be poisoned when the amount of template is high, and the exponential amplification period is longer

Second, choose a hot-start Taq enzyme with strong enzymatic power

The enzymatic motility of taq enzymes is related

Generally speaking, DNA polymerase amplification efficiency is high, the sensitivity is high, but there are also inconsistencies

The amplification sensitivity of Taq enzyme is detected, and the common detection method is to dilute the plasmid fragment of the target gene by 10x or 5x gradient, perform PCR detection at a few lower dilutions, and select the hot-start Taq enzyme

It can be seen that researchers need to choose according to their own experimental requirements and funding, and it is best to do a gradient dilution amplification experiment to detect the amplification efficiency and sensitivity

Cielo^TM real-time PCR system

Harness of the power of qPCR

▌ 12 columns of temperature gradient settings: multi-temperature gradient settings, quickly optimize conditions, determine the appropriate annealing temperature

▌ Good S-type amplification curve

▌ The melting curve is single-peaked, without miscellaneous peaks

In addition, Cielo's™ real-time PCR system combines high-quality Peltier temperature modules with optical inspection systems based on fiber optic transmission to provide highly accurate, sensitive and reliable results

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.