The Vegetable Research Institute of Shandong Academy of Agricultural Sciences has made new progress in the study of salt tolerance in Chinese cabbage

Recently, the molecular breeding team of the Vegetable Research Institute of Shandong Academy of Agricultural Sciences published the "Genome-Wide Identification and Characterization of Chinese Cabbage S1fa Transcription Factors and Their Roles in Response to Salt Stress" research paper
.

 

S1fa transcription factors play an important role
in regulating plant growth and development and responding to processes such as abiotic stress.
Chinese cabbage has four S1fa genes (Bra003132, Bra034084, Bra006994 and Bra029784), and the mechanism of action of these four S1fa genes in response to stress was analyzed
in this study.
The results showed that Bra003132, Bra034084 and Bra029784 were expressed in all tissues of Chinese cabbage, while Bra006994 was expressed only in fruit pods
.
Under Hg and Cd stress, S1fa gene expression increased significantly, while under NaCl stress decreased
significantly.
Further studies have found that overexpression of Bra034084 and Bra029784 genes in yeast species significantly improves the sensitivity
of transgenic yeast to NaCl stress.
The study also found that excessive expression of the Bra034084 and Bra029784 genes significantly reduced the activity of the antioxidant enzymes SOD, POD and CAT, increasing the content
of MDA, H2O2 and O2- 。 In addition, excessive expression of Bra034084 and Bra029784 genes can increase the expression of yeast cell wall biosynthetic genes Ccw14p, Cha1p, Cwp2p, Sed1p, Rlm1p, Rom2p, Mkk1p, Hsp12p, Mkk2p, Sdp1 and YLR194c, while the expression of Bck1p and Ptc1p decreases significantly.
This suggests that the Bra034084 and Bra029784 genes regulate responses
to salt stress by regulating the biosynthesis of cell wall material.
These results lay a foundation
for the in-depth exploration of the molecular regulatory mechanism of salt tolerance in Chinese cabbage and its application in salt tolerance breeding.

 

Fig.
7 Responses to abiotic stresses through yeast dilution bioassay with the wild type strain, S1fa transformed with pRS-416-GFP.
Yeast wild-type （EV） strain and S1fa-overexpressing cells were grown in the URA liquid medium for 24h at 30°C.
The cell solutions were diluted to an OD600value of 0.
3 and exposed to different typesof abiotic stresses.
A.
URA （Control）， B.
50mM Cu, C.
75mM Hg, E.
100 mM Al, D.
100mM Co, F.
75uM Cd, G.
2M mannitol, H.
1M NaCl.
Triangles representthe 10-fold serial dilutions （the starting OD600is0.
3）
。

 

Ali Anwar, a postdoctoral fellow at the Vegetable Research Institute, and Shu Zhang, an assistant researcher, are the co-first authors of the paper, and researchers Gao Jianwei and Dr.
He Lilong are the co-corresponding authors
of the paper.
The research has been funded
by the National Natural Science Foundation of China, the Natural Science Foundation of Shandong Province and the Key R&D Program of Shandong Province.