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The Use of Chemical Genomics to Investigate Pathways Intersecting Auxin-Dependent Responses and Endomembrane Trafficking in Arabidopsis Thaliana

 Last Update: 2021-01-15
 Source: Internet
 Author: User

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Plant endomembrane system is essential for viability and necessary for proper development and signal transduction signal processes. Links between the endomembrane system and auxin signaling have been reported by classical genetics screens. However, the relationship between these processes is not well understood. Chemical genomics is a powerful approach to dissect various processes overcoming lethality and redundancy issues. This approach uses small molecules to modify or disrupt the function of specific proteins and biological processes. We present a screen in
Arabidopsis thaliana
to identify compound affecting auxin-dependent responses and components of the endomembrane system. A gravitropic-response based screen is performed in Arabidopsis seedlings. The identified gravitropic effectors are tested in terms of auxin responsiveness and their effects on endomembrane compartments. These bioactive compounds will be valuable tools for dissecting endomembrane trafficking and auxin signaling processes.

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