The University of Southern California has teamed up with 10X Genomics to develop new single-cell technology that reveals the genetic diversity of cancer.

Introduction: in the past, most tumor cells were sequenced in batches, which allowed researchers to form a general understanding of the genetic composition of tumor tissues.however, this has a limitation: it is impossible to understand the genetic diversity of individual cells.thus, a new single cell technology came into being to solve this problem.the genetic information of a tumor is usually obtained by sequencing millions of tumor cells.although this method provides a broad understanding of the genetic composition of tissues, it may ignore a small number of cancer cells in tumors that are different from most cells.the ability to identify subclones in cancer tissues can provide important biological insights into how cancer develops, how it spreads, and why it is resistant to treatment.due to the limitations of current genome sequencing, it is still a challenge to understand the genetic diversity of individual cells in tumors.researchers at the University of Southern California and 10x genomics, a leader in the single cell sequencing industry, have developed a new technology by using high-throughput single-cell DNA sequencing.in this study, the researchers used an emerging technology called "single cell copy number analysis" developed by 10x genomics, and adopted a novel analysis method, which may provide higher resolution cancer observation that could not be realized before.using a single cell sequencing method based on microdroplets, the researchers sequenced nearly 1500 single cell genomes at the same time, revealing the genetic diversity previously hidden in well studied melanoma cell lines.this study demonstrates the ability of single cell sequencing and reveals the possible evolutionary trajectory of cancer cells. The study is published in nature communication biology.Dr. David Craig, CO director of the Institute of translational genomics at the University of Southern California's Keck School of medicine, said he was surprised that this method was used to examine standard cancer cell lines and carried out thousands of tests in many different laboratories.with this technology, they found unexpected complexity.in fact, this is always a mixture of different types of cells.now that we have these new information, we re-examine our previous work over the past few decades, and they have a new insight into the development of cancer.the researchers analyzed the single DNA of 1475 cells from the colo829 cell line, rather than the tissue DNA of thousands of cells on average.in this high-resolution case, we can find the missing information in low-resolution batch sequencing. this melanoma cell line has previously been characterized by a variety of techniques and serves as a benchmark for evaluating somatic changes. in some previous studies, colo829 showed conflicting or uncertain copy numbers, so single cell sequencing provides a tool to help them observe. their analysis revealed at least four major cell subpopulations (also known as clones), which may be mutated from primitive cancer cells at some stage of cancer cell line evolution. based on clustering, breakpoints, and loss of heterozygosity analysis of aggregated data from subclones, they identified unique landmark events under batch sequencing and spectral karyotype verification. research author and co director of the translational and clinical sciences program at the University of Southern California Norris comprehensive cancer center, and co director of the Institute of translational genomics, University of Southern California If a small number of cells in a tumor have changed and are resistant to treatment, you have to take that tumor out, grind it up and sequence it, and you may not see that change. researchers hope that more cancer researchers will pay attention to single cell sequencing. they also used the technique to study genetic diversity in clinical cancer specimens to better understand the early molecular changes that lead to malignant and refractory advanced cancers. insert an advertisement: single cell sequencing co sponsored by Shanghai Pudong New Area Biological Industry Association, Shanghai Zhangjiang biomedical Base Development Co., Ltd., gene detection technology branch of China Medical Biotechnology Association, genetic diagnosis branch of China genetic society, Shanghai genetic society, Yangtze River Delta integrated gene testing alliance and translational medicine network Clinical application of Technology Salon. time: 13:30-17:30 PM, July 9, 2020 Venue: Zhangjiang · China Pharmaceutical Valley Biomedical Innovation exchange center (first floor, No. 781 Cailun Road, Pudong New Area, Shanghai) is free of charge, welcome to pay attention! 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