The three details are well grasped, and the success rate of human iPS-liver differentiation is up up up!

The importance of the liver is self-evident.

Hepatocytes differentiated from human iPS cells have similar functions to primary hepatocytes and retain the genetic background of the donor.

Takara Cellartis iPS Cell to Hepatocyte Differentiation System (Y30055) is a complete, efficient and novice-friendly differentiation system that can generate functional human iPS-derived hepatocytes within three weeks

Come on, video! In 6 minutes, quickly learn about Takara's iPS-liver differentiation system (Y30055) !

The essence of the details is a serious attitude and a scientific spirit, and the mystery lies in the details

1.

Using the right seeding density is the key to successful iPS-liver differentiation, and it is important to count the cells correctly

In the differentiation stage of iPS-DE cells , too sparse initial iPS cells will lead to a large number of cell deaths before day 4 of differentiation; too dense will result in reduced homogeneity during the differentiation process; the recommended seeding density is 4.

In the differentiation stage of DE-hepatocytes , too sparse initial DE cells will result in a shortened survival and culture cycle and poor performance of the obtained hepatocytes, because the survival and function maintenance of hepatocytes require cell-to-cell contact; if it is too dense, the excess cells will not Will stick to the wall and will be removed when changing the liquid; the recommended inoculation density is 1.

2.

During the differentiation stage of iPS-DE cells, change the medium at about the same time every day , try to be from 10 am to 4 pm, to ensure that the cells continue to provide fresh nutrients and growth factors

3.

In the differentiation stage of DE-hepatocytes , the preparation, formation and subsequent replacement of the overlay layer (Overlay) are very important

When changing the liquid on Day 14 and Day 16, make sure that the temperature of the Maturation Medium is between 16°C and 18°C

Do not move and touch the cells within 48 hours of changing the Maturation Medium

When changing the liquid on Day 18 and the following days, do not damage the covering layer

If you have any questions, please contact us for consultation (Ms.

Attached "Takara People iPS-Hepatic Differentiation System (Y30055) Information Collection" , take it away, you are welcome!

1.
Operating instructions: Cellartis iPS Cell to Hepatocyte Differentiation System User Manual
2.
Technical information: A simple and complete system to generate mature hepatocytes from various iPS cell sources
3.
Technical information: Next-generation human iPS cell-derived hepatocytes for metabolic disease modeling
4.
Technical information: Next-generation human iPS cell-derived hepatocytes for long-term drug metabolism studies
5.
Application literature: Cellartis iPS Cell to Hepatocyte Differentiation System citation list

For more details, please click:
Takara Bio China official website