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    Home > Biochemistry News > Biotechnology News > The selection of bovine serum albumin (BSA) in the trial, different levels.

    The selection of bovine serum albumin (BSA) in the trial, different levels.

    • Last Update: 2020-10-26
    • Source: Internet
    • Author: User
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    customers often encounter such problems, their own experiments need to be used Cowsserum albumin (BSA), if there is no previous targeted supplier, it is estimated that you need to go online to search for suppliers

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    but the information on the Internet is mostly ambiguous, there is ox serum white protein, There are also bovine serum albumin v parts, many suppliers are not clear about its differences, which brings great inconvenience to scientific research users, buy the wrong may be a waste of money, time and energy.


    Company because of its long history of supply of bovine serum albumin, according to personal and colleague experience, summarized the following points for you to share, there are wrong places to look up to people, not very grateful!

    1, using a rating of


    < p-style-text-align:left;"> cow serum albumin grade from low to high:


    cow serum albumin,

    .


    cattle serum albumin fifth group,


    national serum albumin (no fatty acids required),


    ornate serum albumin (protease-free),


    cattle serum albumin (cells culture level),

    .


    gold-grade bovine serum albumin,



    2, BSA at all levels Description


    1, the lowest grade BSA, this is the general sense of BSA, as long as the main component is BSA products can become bovine serum albumin, so he also covers other grades of BSA, so customers should pay attention to its purity.

    : Generally for industrial production.


    2, bovine serum albumin fifth group, English name: BSAV, this is biotechnology Level of products, the widest range of applications, some manufacturers of BSAV fatty acids and endotoxins are very low, can be directly used incell culture, high purity can even be used in any related experiments. Commonly used are Roche's 10735078001, Sigma's A7906, Amresco's 0332.


    effect: Elisa experiment, WB experiment, because the BSA molecular weight is 66KDa, high purity can also be used asproteinmarker.


    3, bovine serum albumin (no fatty acids required), very low fatty acid content, fatty acid content ≤0.002% (w/w), or fatty acid concentration in 1% BSA solution 2 smol/L, e.g. BSA with Equitech bio number BAH66 (our company has a long-term off-the-shelf supply).


    effect: can be used to determine the concentration of free fatty acids released by cultured tissue cells, etc.

    4, bovine serum albumin (no protease), or Protease-free bovine serum albumin, or Protease Free BS A, products do not contain DNAse enzymes, proteases and IgG, such as Equitech bio company number BAH67 BSA (our company has long had off-the-shelf supply) and wako cargo number: 010-15131 bovine serum protein.

    effect: used for radiation immunity, enzymatic immunity, and as a diluent or closure agent.

    5, bovine serum albumin (cell culture level), purity 98%, but requires fatty acid content and Endotoxin content is very low, such as: sigma number A8806 BSA (long-term out of stock abroad, there is no spot at home) and Equitech bio number BSA (our company has long-term spot supply).

    : experiments related to cell culture and cell culture, etc.

    6, gold-grade bovine serum albumin, high purity, purity of more than 99%.

    effect: making gold labels antibodyetc.

    7, cross-linked bovine serum albumin: frozen Dryingpowders, including monomer (66000), dMOs (132000), trimers (198000), tymogens (264000), etc.

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    effect: to determine the molecular weight of unknown proteins, can also be used forbio-chemical teaching experiments to explore electrophoresis conditions, check electrophoresis results, etc.

    wako cargo number: 010-15094 crystalline bovine serum protein, which is the highest purity BSA, can be used as " standard

    (Responsible Editor: King Kunlun of Great Han)

    .
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