The relationship between protection of retinal ischemia-reperfusion injury and autophagy by Ginkgo biloba extract

Recently, researchers from the pharmacology department of Shanghai Medical College published a paper to study the effect of Ginkgo biloba extract (GBE) pretreatment on autophagy after retinal ischemia-reperfusion injury in rats, and to explore the mechanism of GBE protecting retinal ischemia-reperfusion injury It is suggested that GBE has protective effect on neurons after retinal ischemia-reperfusion injury in rats, and its mechanism may be related to the up regulation of Beclin1 and MAP1-LC3 This article was published in the 5th issue of Journal of Shanghai Jiaotong University Medical Edition in 2014 The experimental rats were randomly divided into five groups: normal control group, ischemia-reperfusion model group, GBE low-dose (1 mg / kg) intervention group, GBE medium dose (3 mg / kg) intervention group and GBE high-dose (10 mg / kg) intervention group Min method was used to make the experimental retinal ischemia-reperfusion injury model, to observe the thickness of the inner reticulum layer (IPL), the inner core layer (inl) and the number of ganglion cell layer (GCL) cells in the rat retina; TUNEL method was used to detect apoptosis cells in the retina; immunohistochemistry and Western blotting were used to detect the expression of autophagy related proteins LC3 and Beclin1 Compared with the model group, GBE (3 and 10 mg / kg) intervention group can significantly improve the IPL, inl and GCL damage caused by ischemia-reperfusion injury, reduce the retinal damage (P < 0.05), reduce the apoptosis rate of retinal cells (P < 0.01), increase the number of LC3 and Beclin1 positive cells in retina (P < 0.01), and up regulate the expression of LC3 and Beclin 1 protein in ischemic retina.