The Protein Platform Bioimaging Center cooperated with Thermo Fisher Scientific to realize the integral differential phase contrast...

On February 3, 2022, the Bioimaging Center of the Protein Science Research Platform of the Chinese Academy of Sciences and Thermo Fisher Scientific jointly published an online publication entitled "Imaging biological samples by integrated differential phase" in the journal " Journal of Structural Biology " Contrast (iDPC) STEM technique", reported the application of a new scanning transmission electron microscopy imaging technique-integrated differential phase contrast imaging (integrated differential phase contrast) in biological samples, which is the technology in the ultra-thin bio-resin The first exploration in slices

The picture shows the schematic diagram of the STEM imaging process and the TEM and iDPC-STEM images

Scanning Transmission Electron Microscopy (STEM) is a powerful imaging technique that has been widely used in materials science research.

In recent years, the integrated differential phase contrast imaging (iDPC) technology has emerged.

The picture shows the images of TEM and iDPC-STEM with low dose, reduced staining and thick samples, and the comparison of signal-to-noise ratio of TEM, ABF-STEM, and iDPC-STEM

TEM, iDPC-STEM, and ABF-STEM are also compared in the article, and it is found that iDPC-STEM maintains the high-frequency biological structural details, while moderately improved in the lower frequency domain, and the image contrast is further improved

Researcher Fei Sun of the Institute of Biophysics, Chinese Academy of Sciences and Ivan Lazic of Thermo Fisher Scientific are the corresponding authors of the paper, and Li Xujing, an engineer at the Bioimaging Center, and Huang Xiaojun, a senior engineer, are the co-first authors

Article link: https:// align="justify"> 

(Contributed by: Center for Bioimaging)