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Relying on the fish gene resources and data resources preserved by the Pearl River Branch of the National Freshwater Aquatic Germplasm Resource Bank (affiliated to the Pearl River Fisheries Research Institute of the Chinese Academy of Fishery Sciences), the direct homologous genes were screened from the genomic data of 131 fish species, and 82 fish universal nuclear gene molecular markers (FishPIE) were initially developed through further screening of PCR primer specificity and PCR amplification efficiency of 203 species of 31 orders.
A phylogenetic tree of 710 species of fish in 60 orders, 190 families, and 60 orders were constructed, with a resolution comparable to
that of phylogenetic studies using hundreds of markers.
The development and application of new markers can realize the rapid classification and identification of fish germplasm, reduce the time-consuming, laborious and costly problems of existing technology under the premise of ensuring accuracy, and at the same time provide a new scheme for fish phylogeny, and enrich the basic data
of fish phylogeny research based on molecular markers.
A phylogenetic tree of 710 species of fish in 60 orders, 190 families, and 60 orders were constructed, with a resolution comparable to
that of phylogenetic studies using hundreds of markers.
The development and application of new markers can realize the rapid classification and identification of fish germplasm, reduce the time-consuming, laborious and costly problems of existing technology under the premise of ensuring accuracy, and at the same time provide a new scheme for fish phylogeny, and enrich the basic data
of fish phylogeny research based on molecular markers.
The research work has been supported
by the National Key Research and Development Program of China (2018YFD0900705), the China-ASEAN Maritime Cooperation Fund (CAMC-2018F)), and the National Freshwater Aquatic Germplasm Resource Bank (FGRC18537).
Mou Xidong, Yang Yexin and Sun Jinhui are co-first authors, and Mou Xidong and Ma Jiaxin of Sun Yat-sen University are co-corresponding authors
.
by the National Key Research and Development Program of China (2018YFD0900705), the China-ASEAN Maritime Cooperation Fund (CAMC-2018F)), and the National Freshwater Aquatic Germplasm Resource Bank (FGRC18537).
Mou Xidong, Yang Yexin and Sun Jinhui are co-first authors, and Mou Xidong and Ma Jiaxin of Sun Yat-sen University are co-corresponding authors
.
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