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Figure: Can also be used to study the spectrum of pneumococcal resistance without isolating strains (pneumococcal bacteria observed under the microscope)
A study published in the journal PLOS ONE could one day help health workers determine whether bacteria of the type streptococcus pneumoniae are resistant to antibiotics, which cause meningitis — inflammation
This type of analysis is not an easy task when using traditional methods
Researchers at the Santa André branch of the Adolfo Lutz Institute (IAL) in Brazil have developed a highly feasible new approach
As part of the lab's routine work, the scientists analyzed samples using real-time PCR (real-time PCR), the gold standard
They then reanalyzed the pneumococcal test-positive sample to detect three genes associated with antibiotic resistance, again using real-time PCR, but this time using SYBR Green, a dye that binds to DNA and emits a fluorescent signal that is captured
To find out how resistant bacteria are to antibiotics like penicillin, lincoamide, or macrolides, they used the understanding of the off-curve technique
After completing all these steps, the researchers compared
"We found that 51% of the samples received by IAL between 2014 and 2020 were sensitive
In addition, Streptococcus pneumoniae is able to alter its genetic makeup during the reproductive process, allowing new copies to have genes
The study was supported
essay
Multiplex real-time PCR using SYBR Green: Unspecific intercalating dye to detect antimicrobial resistance genes of Streptococcus pneumoniae in cerebrospinal fluid
