The National Center for Edible Bacteria's medicinal platform made progress in the study of lily to promote the absorption activity of Caco-2 cell glucose

The results were published in food and Chemical Toxicology (IF:4.679, JCR TOP Q2) under the title "Glucose absorption regulation and mechanism of the compounds in Lilium lancifolium Thunb on Caco-2 cells" The chemical composition of Lilium lancifolium Thunb mainly includes saponins, alkaloids, flavonoids, glycolic acids and polysaccharides, and pharmacological activities include antibacterial, blood lipid, anti-inflammatory and antioxidant.
the study group isolated compounds from collibends: saffol, coffee acid, and quercetin-3-O-β-D-semi-lactose glycoside.
literature research found that there were more studies on the pharmacological activity of paracetamol, coffee acid and quercetin-3-O-β-D-semi-lactose glycoside, such as anti-inflammatory, blood lipid, antioxidant and antidepressant.
no paracetamol, coffee acid, and quercetin-3-O-β-D-semi-lactose glycosides based on Caco-2 cell glucose absorption reports.
, the three compounds isolated in the coco-2 cells were studied in this paper to regulate and act on the absorption of Caco-2 cell glucose.
results show that cultured Caco-2 cells can naturally differentiate into similar morphological and bio-chemical characteristics to mature small intestine cells.
at this time Caco-2 cells can be used as in-body models for studying small intestine absorption and are being cultured to 15-16 d.
coffee acid and quercetin-3-O-β-D-semi-lactose glycoside have the effect of promoting the absorption of glucose by Caco-2 cells.
no significant effect on glucose content in Caco-2 cells.
fluorescent D-glucose congeners (2-NBDG) to trace the effect of compounds on glucose intake and consumption of Caco-2 cells, inverted fluorescence microscope photography, 2-NBDG intake is directly related to fluorescence intensity, to measure The sugar intake capacity of Caco-2.
basis, the relevant signaling paths SGLT1 and GLUT2 of Caco-2 cell glucose absorption are further explored.
explains the mechanism by which coffee acid and quercetin-3-O-β-D-semi-lactose glycoside promote the absorption of glucose in Caco-2 cells.
above studies have found that coffee acid and quercetin-3-O-β-D-semi-lactose glycoside promotes the absorption of Caco-2 cell glucose by facilitating transcription of SGLT1 and GLUT2 mRNA and expression of its proteins.
the team conducted a series of studies on glucose digestion, absorption and transport, resulting in a proven research strategy, published in the journal Future Media Chemistry (); International Journal of Biological Macromolecules (); Food Science and Human Wellness; Biomedicine and Pharmacotherapy (); Food Chemical and Chemical () and others.