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    Home > Active Ingredient News > Drugs Articles > The method and correct placement of electric pipette for pipetting

    The method and correct placement of electric pipette for pipetting

    • Last Update: 2022-08-20
    • Source: Internet
    • Author: User
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    The electric pipette is used to transfer a small amount of liquid, including 20-200ul, 200-1000ul, 100-1000ul, and 1-10ml and other specifications.
    It can greatly improve your work efficiency during the experiment, which is both accurate and fast.
    , really a small laboratory equipment
    .

    But there are many precautions in its use, you all know? The editor has sorted it out, everyone can take a seat and study hard
    .

    Before pipetting, ensure that the pipette, tip and liquid are at the same temperature
    .

    When aspirating the liquid, keep the pipette upright and insert the pipette tip 2-3 mm below the liquid surface
    .

    Wet the tip by aspirating the liquid a few times before aspirating (especially if you are picking up liquids that are viscous or have a density different from water)
    .

    There are two pipetting methods available at this time
    .

    One is forward pipetting
    .

    Press the button with your thumb to a stop, then slowly release the button to return to the origin
    .

    Then press the button to the first stop to discharge the liquid, stop for a while and continue to press the button to the second stop to blow out the remaining liquid
    .

    Then release the button
    .

        The second is the reverse pipetting method
    .

    This method is generally used to transfer high-viscosity liquids, biologically active liquids, easily foaming liquids or trace amounts of liquids
    .

        First press the button to the second stop, then slowly release the button to the origin
    .

    Then press the button to a stop point to discharge the liquid with the set range, continue to hold the button at a stop point (never press it down again), remove the pipette tip with residual liquid, and discard it
    .

        After use, it can be hung upright on the pipette holder, but be careful not to drop it
    .

    When there is liquid in the pipette tip, do not place the electric pipette horizontally or upside down to prevent the liquid from flowing back and corroding the piston spring
    .

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