The José C. Pastor-Pareja group of the School of Life Sciences reported on the structure of the endoplasmic reticulum-Golgi apparatus in Drosophila

Protein secretion is essential for the morphogenesis and physiological processes of eukaryotes

On September 14, 2021, the José C.

Figure 1.

This study mainly used transgenic Drosophila, three-dimensional structural illumination microscopy, transmission electron microscopy, and focused ion beam scanning electron microscopy (FIB-SEM) techniques to describe the structure of the Drosophila endoplasmic reticulum-Golgi unit, in which the vesicle protein is endoplasmic The net exit (ERES)-Golgi interface presents a COPII center/COPI circumferential distribution, which is similar to the previous report in Pichia pastoris [2].

Figure 2.

In addition, three-dimensional reconstruction revealed that there are a large number of vesicles and budding structures in the collagen-secreting fat body and the endoplasmic reticulum-Golgi region in the wing disc, and the diameter of the vesicles shows a bimodal distribution (Figure 3).

Figure 3.

This work uses genetically modified fruit flies and high-resolution imaging technology to describe in detail the structural composition of the Drosophila fat body and wing disc endoplasmic reticulum-Golgi unit.

Researcher José C.

Original link: http://doi.

José Carlos Pastor-Pareja Laboratory: http://joselab.

Further reading:

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Weigel, AV, Chang, CL, Shtengel, G.
, Xu, CS, Hoffman, DP, Freeman, M.
, Iyer, N.
, Aaron, J.
, Khuon, S.
, Bogovic, J.
, et al .
(2021).
ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER.
Cell 184, 2412-2429 e2416.