The Jie Wei research group in the School of Life sciences and its collaborators revealed the map of the early human embryonic translation group and found that TPRXs are involved in human...

In the oocyte-to-embryo transition (OET) of mammalian eggs, translation plays an important role

The Ribo-RNA-lite (R2-lite) method developed by combining ultra-sensitive translation group sequencing technology Ribo-lite (Ligation-free, ultra-low-InpuT and Enhanced Ribo-seq) and transcriptome sequencing technology (Smart-seq2) can be used to combine translation group and transcriptome sequencing, which has been applied to human eggs and early embryos for a total of 8 periods

Early human embryonic translation group data also show that a set of Homeobox transcription factors exhibit high translational activity near the activation phase of the human zygote genome, and that their potential DNA binding motif is highly enriched

The article was published in length form on September 8, 2022 in the journal Science, titled "Translatome and Transcriptome Co-profiling reveals a role of TPRXs in human zygotic genome activation

Professor Jie Wei of Tsinghua University, Academician Chen Zijiang of Shandong University and Professor Zhao Han are the co-corresponding authors of this article, Zou Zhuoning, a 2018 doctoral student at Tsinghua-Peking University Life Science Joint Center, Zhang Chuanxin, a 2021 doctoral student at the Research Center for Reproductive Medicine of Shandong University, Wang Qiuyan, a 2020 doctoral student at the School of Life Sciences of Tsinghua University, and Hou Zhenzhen, assistant researcher of the Research Center for Reproductive Medicine of Shandong University, are the co-first authors

Thesis link:

Combined sequencing of human egg and early embryos by translation group with transcriptome reveals translational regulatory mechanisms and key factors for zygonomic activation