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Jasmonic acid (JA) plays an important regulatory role
in plant defense and growth and development.
As an important regulatory node of the JA signaling pathway, the transcription factors of the MYC2 family regulate JA after the plant receives the JA signal In response to the expression of genes and participated in the regulation of stress resistance, secondary metabolite synthesis, growth and development, etc
.
Since 2017, multiple laboratories have utilized the same EMS-induced jin1-2/myc2 The study of related mutants found that the MYC2 family transcription factor significantly negatively regulated the flowering time of Arabidopsis, which was considered to be a key control node that coordinated the resistance and flowering development mediated by the JA pathway.
Some in vitro experiments have shown that these MYC transcription factors may bind to flowering encode FT gene promoters and inhibit their expression, thereby affecting flowering time
.
However, other MYC2-related mutants such as MYC2-2 and MYC2-3 have been used in previous laboratories However, the research carried out shows that the role of MYC family transcription factors in regulating flowering time is limited; FT is also not included in the list of potential target genes regulated by transcription factors in the MYC family.
Therefore, whether and to what extent the JA pathway MYC transcription factor is involved in the regulation of plant flowering timing remains controversial
。
Recently, Hu Jinyong's team at the Kunming Institute of Botany, Chinese Academy of Sciences, and the team of Professor Cui Haitao of Fujian Agriculture and Forestry University, based on a series of rigorous genetics and biochemical and molecular biology experiments, found to be hidden in the widely used JIN1-2/MYC2-related mutants There is a unique mutation in the COP1 gene (named COP1-21 ）
。 COP1 is a key gene for important biological processes such as photomorphogenesis and flowering time regulation, encoding an E3 ubiquitin ligase
。 This mutation causes serine (S) at position 648 of the COP1 protein to become asparagine (N), which is severely affected The interaction potential of COP1 and the trans regulator CONSTANS (CO) upstream of FT was described , leading to accumulation of CO proteins; The increase in CO systematically increases FT expression, so that Arabidopsis thaliana that grows even under short-day conditions has the ability to grow in long-day sunlight FT expression patterns and higher basal levels led to significant early flowering of Arabidopsis thaliana (Fig.
1 and Fig.
2).
。
The study cleared up years of confusion about the regulation of flowering time by MYC transcription factors, and also discovered COP1-21, an important new genetic material
。 Studies in recent decades have shown that COP1 can Binding to different substrate protein molecules leads to ubiquitination degradation of their interacting proteins, which in turn regulates a series of biological processes
.
The COP1-21 mutation is located within the last WD40 domain of COP1, with the currently commonly used missing WD40 domain COP1-4 is different from COP1-6, which occurs with same-frame insertion mutations prior to the WD40 domain, COP1-21 The mutant has no obvious developmental defects, thus providing important genetic resources
for further in-depth analysis of COP1 function.
Recently, a hidden mutation in the seventh WD40-repeat of COP1 determines the early flowering trait in a set of Arabidopsis myc mutants Published online in Plant Cell
.
The reviewers considered "This study clarified the significant role of COP1 and corrected the current understanding of MYC2 in the regulation of flowering, and it should be interesting to the general.
" audience”，“This is a valuable finding.
.
.
.
.
.
”
。 Dr.
Yu Dongmei of the Kunming Institute of Botany, Chinese Academy of Sciences carried out most of the genetics and some biochemical experiments, and was the first author
of the article.
Dr.
Dong Xue used BSA method to discover COP1-21 mutations and participated in some data analysis, Jiang Xiaodong, Sun Yibo, Min Zhijie, Zhang Liping participated in some genetics and molecular experiments, and Zou Ke of Fujian Agriculture and Forestry University participated in some biochemical experiments and was a co-author
of the paper.
Professor Cui Haitao of Fujian Agriculture and Forestry University, together with researcher Hu Jinyong and Dr.
Dong Xue, are co-corresponding authors
.
The research was supported by the National Natural Science Foundation of China and the Chinese Academy of Sciences, and was also partially supported
by the Southwest China Wildlife Germplasm Resource Bank and the Key Laboratory of East Asian Plant Diversity and Biogeography of the Chinese Academy of Sciences with the help of platforms such as plant multidimensional imaging and diversity analysis.
Link to the article
Figure 1 Commonly used hidden COP1-21 in the jin1-2/MYC2 mutant Causes early flowering of Arabidopsis
Fig.
2 Molecular biochemical mechanism of COP1-21 early flower
