The first gene targeting technology to produce transgenic sheep in China

Introduction: four transgenic cloned sheep were born in Qingdao, Shandong Province not long ago This is the first success of functional gene transgenic sheep in China The application of gene targeting technology in goats is the first in the world It is reported that the first four transgenic cloned sheep were born successfully from September 28 to October 7, 2002 Among them, Xiang Xiang (male) and Xiang Xiang (female) were transformed into antithrombin Ⅲ (antithrombin Ⅲ) gene sheep, Shanshan (female) into β - interferon gene sheep, and the other one died 25 hours after birth DNA detection showed that the prospective sheep cloned by transforming antithrombin Ⅲ and interferon β gene had been obtained in this experiment At present, three transgenic cloned sheep are growing well, the weight of ram has reached 11.8kg, and the weight of Xiangxiang and Shanshan are 9kg and 7.5kg respectively Transgenic cloned goat mammary gland bioreactor project is a high-tech bioengineering project with international advanced level It integrates the latest achievements in the fields of genetic engineering, transgenic animals, animal cloning, protein engineering and bioinformatics Its research purpose is to use molecular biology technology to integrate important medical protein genes with those in goat mammary gland Through cell culture, transfection and cloning, a certain protein gene is linked to obtain transgenic cloned goat, which can produce functional protein for human health care and disease treatment at the same time of goat lactation by using the characteristics of high milk yield and protein content.