The effect and mechanism of the mixture of Astragalus and Rehmannia on neovascularization
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Last Update: 2014-07-07
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Source: Internet
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Author: User
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Recently, researchers from the Pharmaceutical Department of Mawangdui sanatorium in Hunan Province published a paper to observe the effect of Astragalus and Rehmannia on the angiogenesis of human umbilical vein endothelial cells, and preliminarily explore the mechanism It is suggested that Astragalus and Rehmannia can induce endothelial cell proliferation, migration, angiogenesis and promote angiogenesis, which may be related to vegf-vegfr-2 pathway This article was published in the 3rd issue of Journal of Wuhan University (Medical Edition) in 2014 The mixed extracts of Astragalus and Rehmannia were 18.75, 37.5 and 75 mg / L respectively The proliferation of endothelial cells was detected by MTT method The migration ability of endothelial cells was examined by scratch test and Transwell test The formation of new blood vessels was observed by tubule formation test The total protein and phosphorylation expression of VEGFR2, Akt, p38 and FAK were determined by immunoblotting The drug concentration of 18.75mg/l can not improve the ability of angiogenesis in vitro; 37.5mg/l can promote the proliferation and angiogenesis of endothelial cells, and can significantly increase the expression of p-fak and p-vegfr-2, but can not promote the migration of endothelial cells; 75mg / l can promote the proliferation, migration and angiogenesis of endothelial cells, and can significantly increase the expression of p-Akt, p-fak and p-vegfr-2.
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