The difference between traditional handmade glue and prefabricated glue

Polyacrylamide and agarose are two commonly used support matrices for electrophoresis, which are porous media that behave like molecular sieves

Buffer systems for electrophoresis are carried out in a continuous or discontinuous manner

A polyacrylamide gel containing a single concentration is called a fixed concentration gel, and a gel with a gradient range of polyacrylamide gel concentration is called a gradient gel

The original SDS polypropylene gel is tubular

Bio-rad's Mini Manual Polyacrylamide Gel Casting System

Usually Mini-type handmade polyacrylamide gels are poured in two glass plates of about 8 cm X 10 cm fixed with supports.

First of all, the PH value of the separating gel is 8.

Secondly, acrylamide is a neurotoxic substance.

Once again, manually pouring the gel is basically a fixed concentration gel.

Finally, alkaline conditions cannot guarantee the stability of some proteins.

Based on the above points, another kind of prefabricated polyacrylamide gel with buffer system appeared on the market as a commercial product

First of all, the buffer system in the gel is Bis-Tris-Cl system, and the pH value is 6.

Secondly, as a prefabricated gel, it no longer needs to be manually poured, avoiding contact with acrylamide-type toxic substances

Once again, as a commercial precast gel, there are different concentrations and gradients to choose from.

The Future PAGE™ brand precast gels represented by NoninBio are based on the Bis-Tris buffer system

Future PAGE™ Precast Gel Products

Future PAGE™ exhibits excellent separation performance for samples with high impurity content