New methods for the safe induction of pluripotent stem cells have been "cracked"
Recently, Yang Xin's team from Cardiff University in the United Kingdom and Wuhan Ruijian Pharmaceutical team published an article
In this way, deviations
It is reported that the research results originated from the "EPSRC Industrial Strategy Innovation Placements" program jointly applied by cardiff University School of Medicine and Wuhan Ruijian Pharmaceutical in February 2019, which is mainly committed to the development of new medical sensors for accurate identification of specific biomarkers, in order to achieve early diagnosis of a variety of malignant diseases and the development
At present, with the development of medicine, biotechnology and other disciplines, regenerative medicine has become a disciplinary platform for studying the possibility of curing a variety of diseases, including tissues and organs damaged by age, disease or trauma, as well as congenital defects are expected to be "cured"
The work done by the research team also aims to further explore the application of new technologies
According to the corresponding author of this paper, Professor Yang Xin of the Department of Biomedical Engineering at Cardiff University in the United Kingdom, and Dr.
FPCB uses flexible materials with good mechanical properties and can be used
After the flexible FPCB and the piezoelectric material are formed closely together by physical methods, the SAW can be effectively generated
It is worth mentioning that currently, SAW devices are usually manufactured by lithography processes, such as processing patterned fork finger transducers (IDTs) on piezoelectric substrates such as lithium niobate (LiNbO3).
In order to minimize the strong reliance on cleanroom facilities for the preparation of SAW equipment in future regenerative medicine applications, the research team also explored a completely new technology - FPCB SAW-based cell stimulation (FSCS) devices
The results of the study show that the use of FSCS devices can reduce the stimulation time and be more adapted to the special environment
In addition, in the process of this study, the relevant research team also carried out the working parameters of SAW, which were "tailored" exactly according to the energy required for stem cell differentiation, and formed tens of millions of microwave vibrations per second on the cells to stimulate the activity
Where the interaction between SAW and medium in the apparatus can be explained by numerical and analytical models, the parameters of edge and plane waves from the PDMS-LiNbO3-fluid contact point are determined by the following model:
For SAW boundary conditions, the following equation is determined:
thereinto
New methods for the safe induction of pluripotent stem cells have been "cracked"
Recently, Yang Xin's team from Cardiff University in the United Kingdom and Wuhan Ruijian Pharmaceutical team published an article
In this way, deviations
It is reported that the research results originated from the "EPSRC Industrial Strategy Innovation Placements" program jointly applied by cardiff University School of Medicine and Wuhan Ruijian Pharmaceutical in February 2019, which is mainly committed to the development of new medical sensors for accurate identification of specific biomarkers, in order to achieve early diagnosis of a variety of malignant diseases and the development
of new treatments 。 Previously, the programme was approved and funded by the Uk Research Council, the UK government's highest scientific governing body
.
At present, with the development of medicine, biotechnology and other disciplines, regenerative medicine has become a disciplinary platform for studying the possibility of curing a variety of diseases, including tissues and organs damaged by age, disease or trauma, as well as congenital defects are expected to be "cured"
through regenerative medicine.
In addition, the regenerative medicine platform also provides a new drug screening and toxicological evaluation system for traditional drug development, opening up a new idea
for extensive pharmaceutical development.
The work done by the research team also aims to further explore the application of new technologies
in regenerative medicine.
According to the corresponding author of this paper, Professor Yang Xin of the Department of Biomedical Engineering at Cardiff University in the United Kingdom, and Dr.
Wei Jun, co-founder and CEO of Ruijian Pharmaceutical, the team used the neural differentiation of pluripotent stem cells as the entry point to realize the cell stimulator (FSCS) that produces surface acoustic waves (SAW) by directly clamping the flexible printed circuit board (FPCB) on the piezoelectric substrate, and studied its effect on
the differentiation of human embryonic stem cells (hESCs) into neurons.
FPCB uses flexible materials with good mechanical properties and can be used
under harsh working conditions such as high temperature and high pressure.
The resulting processed cell stimulator can be placed in the incubator for a long time and remains stable
.
After the flexible FPCB and the piezoelectric material are formed closely together by physical methods, the SAW can be effectively generated
.
The so-called SAW is an ultrasound with concentrated energy on the surface, which is particularly suitable for interacting
with surface adherent cells.
By optimizing the control parameters, SAW generates a very high-speed fluid flow in the stem cell medium, which greatly increases
the rate of material transfer between the lytic substances in the culture system and the cells.
It is worth mentioning that currently, SAW devices are usually manufactured by lithography processes, such as processing patterned fork finger transducers (IDTs) on piezoelectric substrates such as lithium niobate (LiNbO3).
In order to minimize the strong reliance on cleanroom facilities for the preparation of SAW equipment in future regenerative medicine applications, the research team also explored a completely new technology - FPCB SAW-based cell stimulation (FSCS) devices
.
The results of the study show that the use of FSCS devices can reduce the stimulation time and be more adapted to the special environment
of cell culture.
Saw's energy and frequency accuracy are also more controllable, and its wavelength is close to the size of the cell, which can directly manipulate
the cell.
In addition, in the process of this study, the relevant research team also carried out the working parameters of SAW, which were "tailored" exactly according to the energy required for stem cell differentiation, and formed tens of millions of microwave vibrations per second on the cells to stimulate the activity
of stem cells.
At the same time, by optimizing the control parameters, SAW generates an extremely high-speed fluid flow in the stem cell medium, which greatly increases
the rate of material transfer between biochemical factors and cells.
Where the interaction between SAW and medium in the apparatus can be explained by numerical and analytical models, the parameters of edge and plane waves from the PDMS-LiNbO3-fluid contact point are determined by the following model:
For SAW boundary conditions, the following equation is determined:
thereinto
