Test steps and principles of CSI-287 anti-dry microbial penetration tester

In recent years, human society has suffered from a number of major public health events, and these dangerous diseases have gradually made medical and health epidemic prevention institutions and personnel realize the importance of safety protection, and cross-infection control in the process of surgery, virus barrier and patient monitoring has also attracted increasing attention
.

Surgical gowns, surgical sheets, and clean gowns have a good barrier effect on contaminated blood and dirt during surgery, which can significantly reduce the occupational exposure risk
of medical staff.

In addition, surgical gowns and surgical sheets can assist in the establishment of a sterile environment and reduce the risk
of intraoperative infection in patients.

Among the requirements of relevant performance indicators, the penetration resistance of microorganisms is one of
the key indicators to evaluate their safety performance.

Blocking the penetration of dry microorganisms refers to the fact that under dry conditions, through the physical synthesis of air movement and mechanical vibration, microorganisms will penetrate the barrier material with dry organic or inorganic particles, such as dwarf carrying germs, cloth fibers and particles in the air
.

Therefore, it is necessary to control the penetration performance of surgical gowns and surgical sheets, and improve the protective performance
of surgical gowns and surgical sheets.

Test principle: 1, the specimen is installed on a specific container, so that the degree of specimen relaxation is kept within the controlled range, above the specimen to add a certain amount of hsf containing Bacillus subtilis, through the pneumatic ball shaker to make the specimen produce a certain frequency of vibration, the staining hsf will penetrate the specimen into the bottom of the Petri dish, and then the Petri dish is counted at constant temperature to obtain the test results
.

2.
When testing, fix the specimens on
a container.

In these containers, multiple containers carrying Bacillus subtilis hsf and 1 container with unstained Hsf added as a control
.

3.
Insert 1 Petri dish
at the bottom of each container close to the bottom of the time.

The device supporting the container is shaken by a gas ball shaker, and all the hsf that penetrates the specimen falls onto the Petri dish, removing the Petri dish culture
.

The experimental results
are evaluated by counting the growing colonies.

Instrument test 1.
Test step analysis: This test is carried out
on the specimen fixed on a container.

Of these containers, 5 containers carrying subtilis hsf served as the experimental group and 1 vessel with unstained hsf added as control
.

Insert a Petri dish at the bottom of each container close to the bottom of the specimen
.

The equipment supporting the container is shaken by a gas ball shaker, and all the hsf that penetrates the specimen falls onto the Petri dish, takes out the Petri dish and cultures
.

Count the number of colonies grown
.

2.
Equipment debugging: ensure that the dry microbial penetration tester is operable
.

(1) Adjust the level according to the level, adjust 4 rubber supports, so that the instrument is in a horizontal position
.

(2) Adjust the frequency of the gas ball oscillator by adjusting the gas ball oscillator so that it reaches a vibration frequency
of 20,800 times per minute.

3.
Sample preparation: (1) Cut 12 pieces to be tested: 12 specimens with a size of 200mm× 200mm
.

(2) Sterilization treatment sterilizes the cut specimens and each test vessel
.

(3) The fixed test container is fixed to the fixed plate through the rubber ring, so that it is closely connected with the fixed plate, and the bottom is on the
stone plate.

(4) hsf is prepared to prepare hsf containing AATCC 9372 spores of Bacillus subtilis, the concentration should be about 108CFU/g, and the preparation process is referred to YY/T 5056.
5-2009
.

4.
Start the trial
.