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Imaging the molecular patterns of cells through fluorescence microscopy or nanomicroscopy may provide insight into the relationship between molecular behavior and function
On the other hand, cells can be physically fixed by quickly freezing into a solid or reaching a temperature of very high viscosity
Researchers at the Max Planck Institute in Germany have developed an ultra-fast cooling device that can be integrated with a microscope, which can achieve ultra-fast freezing and capture of living cells without cryoprotectants during the microscopic observation of living cells, which can be directly used in the fluorescence microscope Observe the molecular activity pattern at any time point (milliseconds) of interest in living cells, allowing the dynamic process of the observation target to be imaged before cryo-capture, and to compare it with precise molecular patterns at multiple scales after cryo-capture of the same cell The imaging is combined with each other, while successfully bypassing the two major problems of molecular motion blur and light destruction
Ultra-low temperature freezing to capture cells is achieved by extremely rapid cooling to an ultra-low temperature (-196°C)-at this temperature molecular motion tends to stop
The researchers directly adhered the microscope glass cover glass (∅ 4 mm) containing the sample to the diamond heat exchanger (∅ 5 mm) and aligned it with the high numerical aperture (NA) air objective lens (NA: 0.
Ultra-fast cryo-capture technology allows the use of high laser power, which is usually destructive, to analyze unseen natural molecular patterns with a resolution of tens of nanometers
"This is an important advancement in fluorescence microscopy, especially the combination of super-resolution microscopy and microscopic spectroscopy, which allows the molecular reactions in cells to be depicted on multiple scales
