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Taking Agilent bioanalyzer as an example, the key points of second-generation sequencing (NGS) quality control are analyzed

 Last Update: 2022-09-14
 Source: Internet
 Author: User

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Second-generation sequencing (NGS) is a technology based on PCR and gene chip technology, developed from first-generation sequencing, which can be sequenced in parallel, with the advantages of

Figure 1 Example of NGS library building and quality control process

Figure 2 Analysis of gDNA using Agilent gDNA ScreenTape assay shows that the higher the DIN value, the better the gDNA quality

Figure 3 RNA integrity analysis using a fragmentation analyzer.

Figure 6 QC

Figure 7 NGS Library QC

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